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Product Info Summary
| SKU: | M02184-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, IHC-F |
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Product info
Product Name
Anti-Hu CD235a Purified GYPA Monoclonal Antibody
SKU/Catalog Number
M02184-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD235a Purified GYPA Monoclonal Antibody (Catalog# M02184-2). Tested in Flow Cytometry, IHC-P, IHC-F application(s). This antibody reacts with Rat, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD235a Purified GYPA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02184-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
JC159
Isotype
Mouse IgG1
Immunogen
Membrane preparation from splenic hairy cell leukemia. The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02184-2 is reactive to GYPA in Human, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
16.3 kDa
Background of GYPA
CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02184-2 is guaranteed for Flow Cytometry, IHC-P, IHC-F Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): 10 μg/ml.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Flow cytometry analysis (surface staining) of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) purified, GAM-APC.
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Immunohistochemistry staining of human adrenal (paraffin-embedded sections) with anti-CD235a (JC159), 5 µg/ml. Commercially tested by LifeSpan BioSciences.
Specific Publications For Anti-Hu CD235a Purified GYPA Monoclonal Antibody (M02184-2)
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