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Product Info Summary
| SKU: | M05953 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Hu CD300a Purified Monoclonal Antibody
SKU/Catalog Number
M05953
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD300a Purified Monoclonal Antibody (Catalog# M05953). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD300a Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05953)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-260
Isotype
Mouse IgG1
Immunogen
HPB human acute lymphoid leukemia cell line. The antibody MEM-260 reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
Cross-reactivity
Cross-reactivity with CLIC5b was not determined.
Reactive Species
M05953 is reactive to CD300A in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
33.2 kDa
Background of CD300A
CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05953 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2 μg/ml.
Western blotting: Positive material: HPB cell line, negative material: JURKAT human cell line, RAJI human cell line; non-reducing conditions.
Validation Images & Assay Conditions
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Separation of human monocytes (red-filled) from CD300a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD300a (MEM-260) purified antibody (concentration in sample 4 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD300a (MEM-260) purified antibody (concentration in sample 4 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD300a Purified Monoclonal Antibody (M05953)
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