Click image to see more details
Product Info Summary
| SKU: | M05953 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hu CD300a Purified Monoclonal Antibody
View all CD300a/LMIR1 Antibodies
SKU/Catalog Number
M05953
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD300a Purified Monoclonal Antibody (Catalog# M05953). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD300a Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05953)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-260
Isotype
Mouse IgG1
Immunogen
HPB human acute lymphoid leukemia cell line. The antibody MEM-260 reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Cross-reactivity with CLIC5b was not determined.
Reactive Species
M05953 is reactive to CD300A in Human
Applications
M05953 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
33.201kDa
Background of CD300a/LMIR1
CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2 μg/ml.
Western blotting: Positive material: HPB cell line, negative material: JURKAT human cell line, RAJI human cell line; non-reducing conditions.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry validation of CD300A using Anti-Hu CD300a Purified Monoclonal Antibody (M05953).
Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD300a (MEM-260) purifiedProtein Target Info & Infographic
Gene/Protein Information For CD300A (Source: Uniprot.org, NCBI)
Gene Name
CD300A
Full Name
CMRF35-like molecule 8
Weight
33.201kDa
Superfamily
CD300 family
Alternative Names
CD300a antigenCMRF35H9; CD300a molecule; CD300a; CLM8; CLM-8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF-35-H9CD300 antigen-like family member A; CMRF35HIRp60; CMRF35-like molecule 8; IGSF12; IGSF12NK inhibitory receptor; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4 CD300A CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9, CMRF35H, CMRF35H9, IGSF12, IRC1, IRC1/IRC2, IRC2, IRp60 CD300a molecule CMRF35-like molecule 8|CD300 antigen-like family member A|CD300a antigen|CMRF35H leukocyte immunoglobulin-like receptor|NK inhibitory receptor|immunoglobulin superfamily member 12|inhibitory receptor protein 60|leukocyte membrane antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD300A, check out the CD300A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD300A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD300a Purified Monoclonal Antibody (M05953)
Hello CJ!
No publications found for M05953
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hu CD300a Purified Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Hu CD300a Purified Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
