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Product Info Summary
| SKU: | M00193-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD38 Purified Monoclonal Antibody
SKU/Catalog Number
M00193-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD38 Purified Monoclonal Antibody (Catalog# M00193-1). Tested in Flow Cytometry, WB, IHC-P, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD38 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00193-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
HIT2
Isotype
Mouse IgG1
Immunogen
Human thymocytes in foetus. The mouse monoclonal antibody HIT2 reacts with an extracellular epitope of CD38, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells. Its binding is blocked by daratumumab.
Reactive Species
M00193-1 is reactive to CD38 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
34.3 kDa
Background of CD38
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00193-1 is guaranteed for Flow Cytometry, IHC-P, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): 10 μg/ml.
Western blotting: 2 μg/ml; positive control: RAJI human cell line, non-reducing conditions.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Separation of human monocytes (red-filled) from CD38 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD38 (HIT2) purified antibody (concentration in sample 2 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD38 (HIT2) purified antibody (concentration in sample 2 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD38 Purified Monoclonal Antibody (M00193-1)
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