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Product Info Summary
| SKU: | M02902 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD49c Purified ITGA3 Monoclonal Antibody
SKU/Catalog Number
M02902
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD49c Purified ITGA3 Monoclonal Antibody (Catalog# M02902). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD49c Purified ITGA3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02902)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
ASC-1
Isotype
Mouse IgG1 kappa
Immunogen
Human SSC-9 cell line (squamous cell carcinoma). The mouse monoclonal antibody ASC-1 recognizes an extracellular epitope of CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02902 is reactive to ITGA3 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
116.6 kDa
Background of ITGA3
CD49c / Integrin alpha 3 is a type I transmembrane glycoprotein proteolytically cleaved into two disulfide linked chains. It noncovalently associates with CD29 (integrin beta 1) to form the VLA-3 complex, an adhesion receptor for extracellular matrix components (fibronectin, laminin 1, laminin 5, entactin, and collagen). It is expressed on adherent cells, mainly on fibroblasts, epithelial cells and endothelial cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02902 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Separation of HeLa cells stained using anti-human CD49c (ASC-1) purified antibody (concentration in sample 16 µg/ml, GAM APC, red) from HeLa cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (surface staining).
Specific Publications For Anti-Hu CD49c Purified ITGA3 Monoclonal Antibody (M02902)
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