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Product Info Summary
| SKU: | M09548-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody
View all beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibodies
SKU/Catalog Number
M09548-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody (Catalog# M09548-1). Tested in Flow Cytometry, WB, IHC-P, IHC-F, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09548-1)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
TB01
Isotype
Mouse IgM
Immunogen
A pool of neuroblastoma cell lines. The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M09548-1 is reactive to B3GAT1 in Human
Applications
M09548-1 is guaranteed for Flow Cytometry, IHC-P, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
38.256kDa
Background of beta-1,3-Glucuronyltransferase 1/B3GAT1
CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Immunohistochemistry: 5-10 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry validation of B3GAT1 using Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody (M09548-1).
Flow cytometry analysis (surface staining) of human buffy coat cells with anti-human CD57 (TB01) purified
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Figure 2. Immunohistochemistry validation of B3GAT1 using Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody (M09548-1).
Immunohistochemistry staining of human adrenal gland (paraffin-embedded sections) with anti-CD57 (TB01)Protein Target Info & Infographic
Gene/Protein Information For B3GAT1 (Source: Uniprot.org, NCBI)
Gene Name
B3GAT1
Full Name
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
Weight
38.256kDa
Superfamily
glycosyltransferase 43 family
Alternative Names
B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); Beta-1,3-glucuronyltransferase 1; CD57; EC 2.4.1.135; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GLCATP; GlcAT-P; GLCATPGLCUATP; GLCUATP; glcUAT-P; Glucuronosyltransferase P; HNK1; LEU7; NK1; NK-1; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase B3GAT1 CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1 beta-1,3-glucuronyltransferase 1 galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1|LEU7 antigen|UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase|glucuronosyltransferase P
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on B3GAT1, check out the B3GAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for B3GAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD57 Purified B3GAT1 Monoclonal Antibody (M09548-1)
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No publications found for M09548-1
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