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Product Info Summary
| SKU: | M02428 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD64 Purified FCGR1A Monoclonal Antibody
SKU/Catalog Number
M02428
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD64 Purified FCGR1A Monoclonal Antibody (Catalog# M02428). Tested in Flow Cytometry, IP, WB, IHC-P, IHC-F, ICC application(s). This antibody reacts with Human, Primate.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD64 Purified FCGR1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02428)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
10.1
Isotype
Mouse IgG1 kappa
Immunogen
Rheumatoid synovial fluid cells and fibronectin purified human monocytes. The mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02428 is reactive to FCGR1A in Human, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
42.6 kDa
Background of FCGR1A
CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02428 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, ICC, WB Boster Guarantee
Recommend Dilution
Flow cytometry: 1-5 μg/ml.
Validation Images & Assay Conditions
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Separation of human monocytes (red-filled) from CD64 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD64 (10.1) purified antibody (concentration in sample 4 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD64 (10.1) purified antibody (concentration in sample 4 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD64 Purified FCGR1A Monoclonal Antibody (M02428)
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