Anti-HUCE1 RRP8 Antibody

Product Name

Anti-HUCE1 RRP8 Antibody

View all HUCE1 Antibodies

SKU/Catalog Number

A09950

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-HUCE1 RRP8 Antibody (Catalog # A09950). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HUCE1 RRP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09950)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human HUCE1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09950 is reactive to RRP8 in Human

Applications

A09950 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

50.715kDa

Background of HUCE1

Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.

Yazaki M., Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
Ishikawa K., DNA Res. 4:307-313(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from MCF-7 cells, using HUCE1 antibody A09950.

Gene/Protein Information For RRP8 (Source: Uniprot.org, NCBI)

Alternative Names

Cerebral protein 1; KIAA0409EC 2.1.1.-; NML; nucleomethylin; ribosomal RNA processing 8, methyltransferase, homolog (yeast); ribosomal RNA-processing protein 8; RRP8 methyltransferase homolog RRP8 KIAA0409, NML ribosomal RNA processing 8 ribosomal RNA-processing protein 8|RRP8 methyltransferase homolog|cerebral protein 1|nucleomethylin|ribosomal RNA processing 8, methyltransferase, homolog

For more info on RRP8, check out the RRP8 Infographic

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In this infographic, you will see the following information for RRP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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