Product Info Summary
SKU: | A02700-Dyl488 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human ADO DyLight® 488 conjugated Antibody
SKU/Catalog Number
A02700-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human ADO DyLight® 488 conjugated Antibody catalog # A02700-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human ADO DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02700-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02700-Dyl488 is reactive to ADO in Human
Applications
A02700-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
29.751kDa
Background of ADO
Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry analysis of A549 cells using anti-Human ADO antibody (A02700-Dyl488).
Overlay histogram showing A549 cells stained with A02700-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human ADO Antibody (A02700-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ADO (Source: Uniprot.org, NCBI)
Gene Name
ADO
Full Name
2-aminoethanethiol dioxygenase
Weight
29.751kDa
Alternative Names
2-aminoethanethiol (cysteamine) dioxygenase; 2-aminoethanethiol dioxygenase; C10orf22; chromosome 10 open reading frame 22; cysteamine (2-aminoethanethiol) dioxygenase (ADO); Cysteamine dioxygenase; DKFZp564C046; EC 1.13.11.19; FLJ14547 ADO C10orf22 2-aminoethanethiol dioxygenase 2-aminoethanethiol dioxygenase|2-aminoethanethiol (cysteamine) dioxygenase|cysteamine (2-aminoethanethiol) dioxygenase (ADO)|cysteamine dioxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADO, check out the ADO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human ADO DyLight® 488 conjugated Antibody (A02700-Dyl488)
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