Anti-Human ADO DyLight® 550 conjugated Antibody

Boster Bio Anti-Human ADO DyLight® 550 conjugated Antibody catalog # A02700-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A02700-Dyl550
Size: 50ug/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

Product Name

Anti-Human ADO DyLight® 550 conjugated Antibody

See all ADO products

SKU/Catalog Number

A02700-Dyl550

Size

50ug/vial

Form

Liquid

Description

Boster Bio Anti-Human ADO DyLight® 550 conjugated Antibody catalog # A02700-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At 2-8?C for one year from date of receipt. Protect from light. Do not freeze.

Cite This Product

Anti-Human ADO DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02700-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A02700-Dyl550 is reactive to ADO in Human

Applications

A02700-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For ADO (Source: Uniprot.Org, NCBI)

Uniprot ID

Q96SZ5

Gene ID

84890

Gene Name

ADO

Full Name

2-aminoethanethiol dioxygenase

Weight

29.751kDa

Alternative Names

2-aminoethanethiol (cysteamine) dioxygenase; 2-aminoethanethiol dioxygenase; C10orf22; chromosome 10 open reading frame 22; cysteamine (2-aminoethanethiol) dioxygenase (ADO); Cysteamine dioxygenase; DKFZp564C046; EC 1.13.11.19; FLJ14547 ADO C10orf22 2-aminoethanethiol dioxygenase 2-aminoethanethiol dioxygenase|2-aminoethanethiol (cysteamine) dioxygenase|cysteamine (2-aminoethanethiol) dioxygenase (ADO)|cysteamine dioxygenase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADO, check out the ADO Infographic

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In this infographic you will see the following information for ADO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-Human ADO DyLight® 550 conjugated Antibody

Question

Please see the WB image, lot number and protocol we used for teratocarcinoma using anti-Human ADO DyLight® 550 conjugated antibody A02700-Dyl550. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-16

Question

I have a question about product A02700-Dyl550, anti-Human ADO DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02700-Dyl550 anti-Human ADO DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-16

Question

Does anti-Human ADO DyLight® 550 conjugated antibody A02700-Dyl550 work on horse Flow Cytometry with sperm?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

Our lab technicians have not validated anti-Human ADO DyLight® 550 conjugated antibody A02700-Dyl550 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Human ADO DyLight® 550 conjugated antibody A02700-Dyl550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse sperm in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-26

Question

Is a blocking peptide available for product anti-Human ADO DyLight® 550 conjugated antibody (A02700-Dyl550)?

D. Zhao

Verified customer

Asked: 2019-06-26

Answer

We do provide the blocking peptide for product anti-Human ADO DyLight® 550 conjugated antibody (A02700-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-26

Question

Would anti-Human ADO DyLight® 550 conjugated antibody A02700-Dyl550 work for Flow Cytometry with teratocarcinoma?

Verified Customer

Verified customer

Asked: 2017-09-13

Answer

According to the expression profile of teratocarcinoma, ADO is highly expressed in teratocarcinoma. So, it is likely that anti-Human ADO DyLight® 550 conjugated antibody A02700-Dyl550 will work for Flow Cytometry with teratocarcinoma.

Boster Scientific Support

Answered: 2017-09-13

Question

I was wanting to use your anti-Human ADO DyLight® 550 conjugated antibody for Flow Cytometry for human teratocarcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human teratocarcinoma identification?

P. Anderson

Verified customer

Asked: 2013-10-02

Answer

You can see on the product datasheet, A02700-Dyl550 anti-Human ADO DyLight® 550 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human teratocarcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-10-02

$330

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A02700-Dyl550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$330.00

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