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Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)
| SKU | MA1074 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human |
| Clonality | Monoclonal |
| Clone Number | DCS-50 |
| Host | Mouse |
| Ig Isotype | Mouse IgG1 |
| Applications | IHC, ICC, WB |
Overview
| Product Name | Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) |
|---|---|
| SKU/Catalog Number | MA1074 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for p16INK4a/CDKN2, cyclin-dependent kinase inhibitor 2A (CDKN2A) detection. Tested with WB, IHC-F, ICC in Human. No cross reactivity with other proteins. |
| Cite This Product | Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Form | Lyophilized |
| Immunogen | Recombinant human p16 protein. |
| Reactivity | Human |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.Images And Assay Conditions
Boster Kit Box
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | Q9R0Z3 |
|---|---|
| Gene Name | CDKN2A |
| Protein Name | Cyclin-dependent kinase inhibitor 2A |
| Tissue Specificity | Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. . |
| Alternative Names | Cyclin-dependent kinase inhibitor 2A ;Cyclin-dependent kinase 4 inhibitor A;CDK4I;p16-INK4a;p16;p16-INK4;Cdkn2a ;P16ink4a ; |
| Subcellular Localization | Cytoplasm . Nucleus . |
| Molecular Weight | 17366 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity). . |
|---|---|
| Research Areas | Human *You can search these to find other products in these research areas. |
| Background | p16(INK4A), also known as cyclin-dependent kinase inhibitor 2A(CDKN2A), or multiple tumor suppressor 1(MTS1). The p16 gene(CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma. |
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Monoclonal clone# G2 antibody for P16INK4A/CDKN2A detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-F. Reactive species: Human. P16INK4A/CDKN2A information: Molecular Weight: 17366 MW; Subcellular Localization: Cytoplasm . Nucleus ; Tissue Specificity: Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain.
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MA1074
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
Publications
Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation
Customer Q&As
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
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Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
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Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
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Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to p19 antibody, arf antibody, cdkn2a antibody