Overview
| Product Name |
Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)
See more Cdkn2a products |
| Catalog# |
MA1074 |
| Pack Size |
100μg/vial |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) catalog # MA1074. Tested in IHC, ICC, WB applications. This antibody reacts with Human. Supplied as 100μg/vial in Lyophilized form antibody. |
| Cite This Product |
Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074)
|
| Antibodies Validation |
Antibodies Validation Information
|
Similar Products From
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Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) may replace the following items: sc 161477|sc 161478|sc 164595|sc 164596|sc 164597|sc 246240|sc 246241|sc 28260|sc 30545|sc 30547|sc 323702|sc 323703|sc 323704|sc 377412|sc 53392|sc 53639|sc 53640|sc 71797|sc 73434|sc 81841|sc 8340|sc 8613. |
Product Specs
| Host |
Mouse |
| Reactive Species |
Human |
| Applications |
IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
Recombinant human p16 protein. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For CDKN2A (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9R0Z3 |
|---|
| NCBI Gene Id | 25163 |
|---|
| Species Of This Entry | Rat |
|---|
| Gene Name | CDKN2A |
|---|
| Protein Name | Cyclin-dependent kinase inhibitor 2A |
|---|
| Superfamily | CDKN2 cyclin-dependent kinase inhibitor family |
|---|
| Alternative Names | Cdkn2a|alternative reading frame; ARF; ARF-INK4a; CDK4I; CDKN2A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a; Ink4a/Arf; INK4a-ARF; mitochondrial smARF'; MTS1; p14; p14ARF; P16; p16(INK4a); p16-INK4; P16INK4A; p16-INK4a; P19; p19<ARF>; p19ARF; Pctr1 |
|---|
| Molecular Weight | 17366 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CDKN2A, check out the CDKN2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for CDKN2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the CDKN2A infographic
Our Boster Quality Guarantee for Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) covers its use in the following applications.
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
| Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation |
PubMed ID 15378766 |
|
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Questions and answers from customers related to MA1074 Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)
13 Related Questions
Question
See attached the WB image, lot number and protocol we used for adenohypophysis using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074. Please let me know if you require anything else.
Verified Customer
Asked: 2020-02-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-24
Question
Does MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Asked: 2020-02-18
Answer
It shows on the product datasheet, MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-18
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074. Let me know if you need anything else.
Verified Customer
Asked: 2020-01-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
We are currently using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Asked: 2019-12-23
Answer
The anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) (MA1074) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Is this MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) reactive to the isotypes of CDKN2A?
Verified Customer
Asked: 2019-11-05
Answer
The immunogen of MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) is Recombinant human p16 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-05
Question
I see that the anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Asked: 2019-10-21
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-21
Question
Will anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 work on monkey WB with adenohypophysis?
Verified Customer
Asked: 2019-07-12
Answer
Our lab technicians have not validated anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey adenohypophysis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
I was wanting to use your anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) for ICC for human adenohypophysis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adenohypophysis identification?
Verified Customer
Asked: 2019-07-12
Answer
You can see on the product datasheet, MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adenohypophysis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
Would anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 work for ICC with adenohypophysis?
Verified Customer
Asked: 2019-05-23
Answer
According to the expression profile of adenohypophysis, CDKN2A is highly expressed in adenohypophysis. So, it is likely that anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 will work for ICC with adenohypophysis.
Boster Scientific Support
Answered: 2019-05-23
Question
My question regards to test anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 on human adenohypophysis for research purposes, then I may be interested in using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Asked: 2019-05-13
Answer
The products we sell, including anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-13
Question
Is a blocking peptide available for product anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) (MA1074)?
Verified Customer
Asked: 2018-01-09
Answer
We do provide the blocking peptide for product anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) (MA1074). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-09
Question
My question regarding product MA1074, anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Asked: 2017-07-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-13
Question
Do you have a BSA free version of anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 available?
M. Gonzalez
Asked: 2016-10-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-10-04
