|Ig Isotype||Mouse IgG1|
|Applications||IHC, ICC, WB|
|Product Name||Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for p16INK4a/CDKN2, cyclin-dependent kinase inhibitor 2A (CDKN2A) detection. Tested with WB, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Recombinant human p16 protein.|
Assay Dilutions Overview
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||Cyclin-dependent kinase inhibitor 2A|
|Tissue Specificity||Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. .|
|Alternative Names||Cyclin-dependent kinase inhibitor 2A ;Cyclin-dependent kinase 4 inhibitor A;CDK4I;p16-INK4a;p16;p16-INK4;Cdkn2a ;P16ink4a ;|
|Subcellular Localization||Cytoplasm . Nucleus .|
|Molecular Weight||17366 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity). .|
|Research Areas||Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics And Nuclear Signaling, Oncoproteins/Suppressors, P53 Pathway, Transcription, Tumor Suppressors
*You can search these to find other products in these research areas.
|Background||p16(INK4A), also known as cyclin-dependent kinase inhibitor 2A(CDKN2A), or multiple tumor suppressor 1(MTS1). The p16 gene(CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma.|
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (MA1074)
Promotion:Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.