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Product Info Summary
| SKU: | MA1074 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)
SKU/Catalog Number
MA1074
BM1592 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) catalog # MA1074. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
DCS-50
Isotype
Mouse IgG1
Immunogen
Recombinant human p16 protein.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1074 is reactive to CDKN2A in Human
Observed Molecular Weight
200 kDa
Calculated molecular weight
17.4 kDa
Background of CDKN2A
p16 (INK4A), also known as cyclin-dependent kinase inhibitor 2A (CDKN2A), or multiple tumor suppressor 1 (MTS1). The p16 gene (CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1074 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, -Immunohistochemistry (Paraffin-embedded Section), 5μg/ml, Human
Western blot, 0.5-1μg/ml, Human
Positive Control
IHC:non-small cell lung cancer
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of CDKN2 using anti-CDKN2 antibody (MA1074).
CDKN2 was detected in a paraffin-embedded section of non-small cell lung cancer. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with mouse anti-CDKN2 Antibody (MA1074) at 5 μg/ml overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Specific Publications For Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) (MA1074)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)
Question
See attached the WB image, lot number and protocol we used for adenohypophysis using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-24
Question
Does MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
It shows on the product datasheet, MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-18
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
We are currently using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) (MA1074) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Is this MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) reactive to the isotypes of CDKN2A?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The immunogen of MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) is Recombinant human p16 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-05
Question
I see that the anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-21
Question
Will anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 work on monkey WB with adenohypophysis?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
Our lab technicians have not validated anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey adenohypophysis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
I was wanting to use your anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) for ICC for human adenohypophysis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adenohypophysis identification?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
You can see on the product datasheet, MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adenohypophysis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
Would anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 work for ICC with adenohypophysis?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
According to the expression profile of adenohypophysis, CDKN2A is highly expressed in adenohypophysis. So, it is likely that anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 will work for ICC with adenohypophysis.
Boster Scientific Support
Answered: 2019-05-23
Question
My question regards to test anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 on human adenohypophysis for research purposes, then I may be interested in using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
The products we sell, including anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-13
Question
Is a blocking peptide available for product anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) (MA1074)?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
We do provide the blocking peptide for product anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) (MA1074). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-09
Question
My question regarding product MA1074, anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-13
Question
Do you have a BSA free version of anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 available?
M. Gonzalez
Verified customer
Asked: 2016-10-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-10-04
