Overview
| Product Name |
Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)
See all Cdkn2a primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
MA1074 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Description |
Monoclonal clone# G2 antibody for P16INK4A/CDKN2A detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-F. Reactive species: Human. P16INK4A/CDKN2A information: Molecular Weight: 17366 MW; Subcellular Localization: Cytoplasm . Nucleus ; Tissue Specificity: Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. |
| Cite This Product |
Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074)
|
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Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) may replace the following items: sc 161477|sc 161478|sc 164595|sc 164596|sc 164597|sc 246240|sc 246241|sc 28260|sc 30545|sc 30547|sc 323702|sc 323703|sc 323704|sc 377412|sc 53392|sc 53639|sc 53640|sc 71797|sc 73434|sc 81841|sc 8340|sc 8613. |
Product Specs
| Host |
Mouse |
| Reactivity/Species |
Human |
| Applications |
IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
Recombinant human p16 protein. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For CDKN2A (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9R0Z3 |
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| NCBI Gene Id | 25163 |
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| Species Of This Entry | Rat |
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| Gene Name | CDKN2A |
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| Protein Name | Cyclin-dependent kinase inhibitor 2A |
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| Superfamily | CDKN2 cyclin-dependent kinase inhibitor family |
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| Alternative Names | Cdkn2a|alternative reading frame; ARF; ARF-INK4a; CDK4I; CDKN2A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a; Ink4a/Arf; INK4a-ARF; mitochondrial smARF'; MTS1; p14; p14ARF; P16; p16(INK4a); p16-INK4; P16INK4A; p16-INK4a; P19; p19<ARF>; p19ARF; Pctr1 |
|---|
| Molecular Weight | 17366 |
|---|
| Post Tranlational Modifications | Phosphoprotein |
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| Gene Location | 5q32, on Chromasome 5, gene sequence: |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CDKN2A (Source: Uniprot.org, NCBI)
| Protein Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity). |
|---|
| Tissue Specificity | Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. |
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| Research Areas | ABC Transporters; Biologically Active Proteins; DNA Repair; Hypoxia; MAP Kinase Signaling; Neuroscience |
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| Background | p16(INK4A), also known as cyclin-dependent kinase inhibitor 2A(CDKN2A), or multiple tumor suppressor 1(MTS1). The p16 gene(CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma. |
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| Scientific References | PMID: 9032263 by Swafford D.S., et al. Frequent aberrant methylation of p16INK4a in primary rat lung tumors. |
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Our Boster Quality Guarantee for Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) covers its use in the following applications.
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all Cdkn2a primary antibodies,
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Total number of citations: 1
| Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation |
PubMed ID 15378766 |
