|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)|
|Description||Mouse IgG monoclonal antibody for p16INK4a/CDKN2, cyclin-dependent kinase inhibitor 2A (CDKN2A) detection. Tested with WB, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) DCS-50 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074)|
|Replacement Item||This antibody may replace the following items: sc-161477|sc-161478|sc-164595|sc-164596|sc-164597|sc-246240|sc-246241|sc-28260|sc-30545|sc-30547|sc-323702|sc-323703|sc-323704|sc-377412|sc-53392|sc-53639|sc-53640|sc-71797|sc-73434|sc-81841|sc-8340|sc-8613 from Santa Cruz Biotechnology.|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Recombinant human p16 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3|
|Molecular Weight||17366 MW|
|Protein Function||Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity). .|
|Tissue Specificity||Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. .|
|Sequence Similarities||Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.|
|Subcellular Localization||Cytoplasm . Nucleus .|
|Alternative Names||Cyclin-dependent kinase inhibitor 2A ;Cyclin-dependent kinase 4 inhibitor A;CDK4I;p16-INK4a;p16;p16-INK4;Cdkn2a ;P16ink4a ;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors|ink4| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|oncoproteins/suppressors|p53 pathway|ink4a/arf||
Background for Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 1μg/ml, Human, -|
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.