| SKU: | MA1074 |
|---|---|
| Pack Size: | 100μg/vial |
| Validated Species: | Human |
| Clonality: | Monoclonal |
| Clone Number: | DCS-50 |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Overview
| Product Name | Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) |
|---|---|
| SKU/Catalog Number | MA1074 |
| Description | Mouse IgG monoclonal antibody for p16INK4a/CDKN2, cyclin-dependent kinase inhibitor 2A (CDKN2A) detection. Tested with WB, IHC-F, ICC in Human. No cross reactivity with other proteins. |
| Cite This Product | Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) DCS-50 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074) |
| Replacement Item | This antibody may replace the following items: sc-161477|sc-161478|sc-164595|sc-164596|sc-164597|sc-246240|sc-246241|sc-28260|sc-30545|sc-30547|sc-323702|sc-323703|sc-323704|sc-377412|sc-53392|sc-53639|sc-53640|sc-71797|sc-73434|sc-81841|sc-8340|sc-8613 from Santa Cruz Biotechnology. |
| Host | Mouse |
| Validated Species | Human |
| Application | IHC, ICC, WB *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Recommended Detection Systems | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC. *Blocking peptide can be purchased at $50. Contact us for more information **Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018! |
| Immunogen | Recombinant human p16 protein. |
| Cross Reactivity | No cross reactivity with other proteins |
| Pack Size | 100μg/vial |
Properties
| Clonality | Monoclonal |
|---|---|
| Clone Number | DCS-50 |
| Form | Lyophilized |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Concentration | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Purification | Ascites |
| Isotype | Mouse IgG1 |
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | Cdkn2a |
|---|---|
| Protein Name | Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3 |
| Molecular Weight | 17366 MW |
| Protein Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity). . |
| Tissue Specificity | Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. . |
| Sequence Similarities | Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. |
| Subcellular Localization | Cytoplasm . Nucleus . |
| Uniprot ID | Q9R0Z3 |
| Alternative Names | Cyclin-dependent kinase inhibitor 2A ;Cyclin-dependent kinase 4 inhibitor A;CDK4I;p16-INK4a;p16;p16-INK4;Cdkn2a ;P16ink4a ; |
| Research Areas | |cell biology|cell cycle|cell cycle inhibitors|ink4| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|oncoproteins/suppressors|p53 pathway|ink4a/arf| |
Background for Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
p16(INK4A), also known as cyclin-dependent kinase inhibitor 2A(CDKN2A), or multiple tumor suppressor 1(MTS1). The p16 gene(CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma.
Related Products
Goat Anti-Mouse IgG Secondary Antibody, HRP Conjugate (BA1054)
Price: FREE if purchase with Primary antibody
Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001)
Price: $100
SuperVision IHC Kit with anti-Mouse IgG (SV0001-1)
Price: $150
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to p19 antibody, arf antibody, cdkn2a antibody
