Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)

Boster Bio Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) catalog # MA1074. Tested in IHC, ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: MA1074
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC, ICC, WB

Product Name

Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)

See all Cdkn2a products

SKU/Catalog Number

MA1074

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) catalog # MA1074. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DCS-50

Isotype

Mouse IgG1

Immunogen

Recombinant human p16 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1074 is reactive to CDKN2A in Human

Applications

MA1074 is guaranteed for IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CDKN2A (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9R0Z3

Gene ID

25163

Gene Name

CDKN2A

Full Name

Cyclin-dependent kinase inhibitor 2A

Weight

17.366kDa

Superfamily

CDKN2 cyclin-dependent kinase inhibitor family

Alternative Names

alternative reading frame; ARF; ARF-INK4a; CDK4I; CDKN2A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a; Ink4a/Arf; INK4a-ARF; mitochondrial smARF'; MTS1; p14; p14ARF; P16; p16(INK4a); p16-INK4; P16INK4A; p16-INK4a; P19; p19<ARF>; p19ARF; Pctr1 Cdkn2a|Arf, INK4A, MTS1, p16, p16Cdkn2a, p19ARF|cyclin-dependent kinase inhibitor 2A|cyclin-dependent kinase inhibitor 2A|CDK4I|Cyclin dependent kinase inhibitor 2A (p16, inhibits CDK4)|alternative reading frame|cell cycle inhibitor|cell cycle regulator|cyclin-dependent kinase 4 inhibitor A|cyclin-dependent kinase inhibitor 2a p16Ink4a|cyclin-dependent kinase inhibitor 2a p19Arf|p16-INK4|p16-INK4a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDKN2A, check out the CDKN2A Infographic

CDKN2A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDKN2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1074 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation

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13 Customer Q&As for Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)

Question

See attached the WB image, lot number and protocol we used for adenohypophysis using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-24

Question

Does MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

It shows on the product datasheet, MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-18

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-29

Question

We are currently using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

The anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) (MA1074) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-23

Question

Is this MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) reactive to the isotypes of CDKN2A?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

The immunogen of MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) is Recombinant human p16 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-05

Question

I see that the anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-21

Question

Will anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 work on monkey WB with adenohypophysis?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

Our lab technicians have not validated anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey adenohypophysis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-12

Question

I was wanting to use your anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) for ICC for human adenohypophysis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adenohypophysis identification?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

You can see on the product datasheet, MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adenohypophysis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-12

Question

Would anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 work for ICC with adenohypophysis?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

According to the expression profile of adenohypophysis, CDKN2A is highly expressed in adenohypophysis. So, it is likely that anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 will work for ICC with adenohypophysis.

Boster Scientific Support

Answered: 2019-05-23

Question

My question regards to test anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 on human adenohypophysis for research purposes, then I may be interested in using anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

The products we sell, including anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-13

Question

Is a blocking peptide available for product anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) (MA1074)?

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

We do provide the blocking peptide for product anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) (MA1074). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-01-09

Question

My question regarding product MA1074, anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-07-13

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1074 anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-07-13

Question

Do you have a BSA free version of anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 available?

M. Gonzalez

Verified customer

Asked: 2016-10-04

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p16INK4a/CDKN2 antibody (Monoclonal, DCS-50) MA1074 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-10-04

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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MA1074
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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