|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)|
|Description||Mouse IgG monoclonal antibody for p16INK4a/CDKN2, cyclin-dependent kinase inhibitor 2A (CDKN2A) detection. Tested with WB, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) DCS-50 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074)|
|Replacement Item||This antibody may replace the following items: sc-161477|sc-161478|sc-164595|sc-164596|sc-164597|sc-246240|sc-246241|sc-28260|sc-30545|sc-30547|sc-323702|sc-323703|sc-323704|sc-377412|sc-53392|sc-53639|sc-53640|sc-71797|sc-73434|sc-81841|sc-8340|sc-8613 from Santa Cruz Biotechnology.|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Recombinant human p16 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3|
|Molecular Weight||17366 MW|
|Protein Function||Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity). .|
|Tissue Specificity||Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. .|
|Sequence Similarities||Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.|
|Subcellular Localization||Cytoplasm . Nucleus .|
|Alternative Names||Cyclin-dependent kinase inhibitor 2A ;Cyclin-dependent kinase 4 inhibitor A;CDK4I;p16-INK4a;p16;p16-INK4;Cdkn2a ;P16ink4a ;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors|ink4| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|oncoproteins/suppressors|p53 pathway|ink4a/arf||