Click image to see more details
Product Info Summary
| SKU: | DZ41311 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC |
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Product info
Product Name
Anti-Human CCNE1 Antibody
SKU/Catalog Number
DZ41311
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-CCNE1 Antibody catalog # DZ41311. This antibody reacts with Human.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-Human CCNE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41311)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ41311 is reactive to CCNE1 in Human
Calculated molecular weight
47.1 kDa
Application & Images
Applications
DZ41311 is guaranteed for IF, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ICC/IF: 1:100-1:200
Validation Images & Assay Conditions
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IF analysis of CCNE1 using anti-CCNE1 antibody (DZ41311).
CCNE1 was detected in an immunocytochemical section of HaCaT cells. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. And then incubated with 1:100-1:200 rabbit anti-CCNE1 Antibody (DZ41311) overnight at 4°C. Goat anti-rabbit IgG conjugated to Alexa Fluor 488 was used as secondary antibody at 1:1000 dilution and incubated for 1-2 hours at RT. The section was counterstained with DAPI. Visualize using Confocal Microscope Zeiss LSM 900 and filter sets appropriate for the label used.
Specific Publications For Anti-Human CCNE1 Antibody (DZ41311)
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Customer Reviews
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1 Reviews For Anti-Human CCNE1 Antibody
A strong nuclear signal for Cyclin E was observed in proliferating cells by IF. Results were reproducible and matched the expected patterns.
Excellent
| SKU | DZ41311 |
|---|---|
| Application | Immunofluorescence |
| Sample | Human HaCaT cell |
| Sample Processing Description | Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked in 1% BSA for 1 hour before incubation with the primary antibody. |
| Primary Antibody | Human CCNE1 Antibody |
| Primary Incubation | 1:100-1:200, overnight at 4 ℃ |
| Secondary Antibody | Goat anti-rabbit IgG conjugated to Alexa Fluor 488 |
| Secondary Incubation | 1:1000, 1-2 hours in room temperature |
| Detection | Fluorescence microscopy using Alexa Fluor 488 (excitation 488 nm, emission 519 nm) Using Confocal Microscope Zeiss LSM 900 |
| Results Summary | The CyclinE-D15 antibody (DZ41311) worked well in IF application using human cell lines. In IF, the nuclear localization of Cyclin E matched the known expression pattern. The antibody showed good specificity with a minimal background. A fluorescent secondary antibody (Alexa Fluor 488) was used for detection and gave strong, clear signal. Overall, a reliable antibody for Cyclin E detection in human cell systems. |
Kasturi Mitra
Verified customer
Submitted 2026-01-06
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