This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CCNE1 antibody (DZ41312) performs well in IF, ICC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IF analysis of CCNE1 using anti-CCNE1 antibody (DZ41312).
CCNE1 was detected in an immunocytochemical section of HaCaT cells. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. And then incubated with 1:100-1:200 rabbit anti-CCNE1 Antibody (DZ41312) overnight at 4°C. Goat anti-rabbit IgG conjugated to Alexa Fluor 488 was used as secondary antibody at 1:1000 dilution and incubated for 1-2 hours at RT. The section was counterstained with DAPI. Visualize using Confocal Microscope Zeiss LSM 900 and filter sets appropriate for the label used.
CCNE1 was detected in an immunocytochemical section of HaCaT cells. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. And then incubated with 1:100-1:200 rabbit anti-CCNE1 Antibody (DZ41312) overnight at 4°C. Goat anti-rabbit IgG conjugated to Alexa Fluor 488 was used as secondary antibody at 1:1000 dilution and incubated for 1-2 hours at RT. The section was counterstained with DAPI. Visualize using Confocal Microscope Zeiss LSM 900 and filter sets appropriate for the label used.
Bundle Save
Anti-Human CCNE1 Antibody
CCNE1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IF, ICC |
Recommend Dilution
Application
Dilution
Species
ICC/IF: 1:100-1:200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Human CCNE1 Antibody
View all CCNE1 Antibodies
View all CCNE1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Calculated Molecular Weight
47.1 kDa
Cite This Product
Anti-Human CCNE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41312)
Review
Have you used Anti-Human CCNE1 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-Human CCNE1 Antibody
A strong nuclear signal for Cyclin E was observed in proliferating cells by IF . Results were reproducible and matched expected patterns.
| SKU | DZ41312 |
|---|---|
| Application | Immunofluorescence |
| Sample | Human HaCaT cell |
| Sample Processing Description | Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked in 1% BSA for 1 hour before incubation with the primary antibody. |
| Primary Antibody | Human CCNE1 Antibody |
| Primary Incubation | 1:100-1:200, overnight at 4 ℃ |
| Secondary Antibody | Goat anti-rabbit IgG conjugated to Alexa Fluor 488 |
| Secondary Incubation | 1:1000, 1-2 hours in room temperature |
| Other Reagents used | PBS, 0.1% Triton X-100, 1% BSA, DAPI for nuclear counterstaining |
| Detection | Fluorescence microscopy using Alexa Fluor 488 (excitation 488 nm, emission 519 nm) Using Confocal Microscope Zeiss LSM 900 |
| Results Summary | The CyclinE-FL antibody (DZ41312) worked in IF application using human cell lines. In IF, the nuclear localization of Cyclin E matched the known expression pattern with good specificity. A fluorescent secondary antibody (Alexa Fluor 488) was used for detection and gave clear signal. Overall, a reliable antibody for full length Cyclin E detection in human cell systems. |
Kasturi Mitra
Verified customer
Submitted 2026-01-06
Product Promise
We promise Anti-Human CCNE1 Antibody performs excellently in relevant applications for IF, ICC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
DZ41312
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026