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Product Info Summary
| SKU: | A01981-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CHRNA3 DyLight® 550 conjugated Antibody
View all Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibodies
SKU/Catalog Number
A01981-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CHRNA3 DyLight® 550 conjugated Antibody catalog # A01981-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CHRNA3 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01981-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CHRNA3, which shares 97.4% and 94.7% amino acid (aa) sequence identity with mouse and rat CHRNA3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01981-Dyl550 is reactive to CHRNA3 in Human
Applications
A01981-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
57.48kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CHRNA3 (Source: Uniprot.org, NCBI)
Gene Name
CHRNA3
Full Name
Neuronal acetylcholine receptor subunit alpha-3
Weight
57.48kDa
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
cholinergic receptor, nicotinic, alpha 3; cholinergic receptor, nicotinic, alpha polypeptide 3; CHRNA3; LNCR2; MGC104879; NACHRA3; neuronal acetylcholine receptor subunit alpha-3; neuronal nicotinic acetylcholine receptor, alpha3 subunit; Nicotinic Acetylcholine R alpha 3; Nicotinic Acetylcholine Ra3; PAOD2 CHRNA3 BAIPRCK, LNCR2, NACHRA3, PAOD2 cholinergic receptor nicotinic alpha 3 subunit neuronal acetylcholine receptor subunit alpha-3|cholinergic receptor, nicotinic alpha 3|cholinergic receptor, nicotinic, alpha 3 (neuronal)|cholinergic receptor, nicotinic, alpha polypeptide 3|neuronal nicotinic acetylcholine receptor, alpha3 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHRNA3, check out the CHRNA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHRNA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CHRNA3 DyLight® 550 conjugated Antibody (A01981-Dyl550)
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No publications found for A01981-Dyl550
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Human CHRNA3 DyLight® 550 conjugated Antibody
Question
I would like to test anti-Human CHRNA3 DyLight® 550 conjugated antibody A01981-Dyl550 on human brain lung for research purposes, then I may be interested in using anti-Human CHRNA3 DyLight® 550 conjugated antibody A01981-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The products we sell, including anti-Human CHRNA3 DyLight® 550 conjugated antibody A01981-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-06
Question
My question regarding product A01981-Dyl550, anti-Human CHRNA3 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-02-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01981-Dyl550 anti-Human CHRNA3 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-11
Question
We are currently using anti-Human CHRNA3 DyLight® 550 conjugated antibody A01981-Dyl550 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
The anti-Human CHRNA3 DyLight® 550 conjugated antibody (A01981-Dyl550) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain lung using anti-Human CHRNA3 DyLight® 550 conjugated antibody A01981-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-04
Question
Does anti-Human CHRNA3 DyLight® 550 conjugated antibody A01981-Dyl550 work on canine Flow Cytometry with brain?
L. Williams
Verified customer
Asked: 2014-12-24
Answer
Our lab technicians have not tested anti-Human CHRNA3 DyLight® 550 conjugated antibody A01981-Dyl550 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Human CHRNA3 DyLight® 550 conjugated antibody A01981-Dyl550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-24
