Anti-Human COPE DyLight® 488 conjugated Antibody(monoclonal, 9B6)

Product Name

Anti-Human COPE DyLight® 488 conjugated Antibody(monoclonal, 9B6)

View all COPE Antibodies

SKU/Catalog Number

M04544-Dyl488

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Human COPE DyLight® 488 conjugated Antibody (monoclonal, 9B6) catalog # M04544-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human COPE DyLight® 488 conjugated Antibody(monoclonal, 9B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04544-Dyl488)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Monoclonal

Clone Number

9B6

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M04544-Dyl488 is reactive to COPE in Human

Applications

M04544-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

34.482kDa

Background of COPE

Coatomer subunit epsilon is a protein that in humans is encoded by the COPE gene. The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry analysis of A431 cells using anti-Human COPE antibody (M04544-Dyl488).
Overlay histogram showing A431 cells stained with M04544-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Human COPE Antibody (M04544-Dyl488,1μg/1x10⁶ cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-Human COPE antibody (M00480-Dyl488).
Overlay histogram showing HL-60 cells stained with M00480-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Human COPE Antibody (M00480-Dyl488,1μg/1x10⁶ cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For COPE (Source: Uniprot.org, NCBI)

Alternative Names

coatomer epsilon subunit; coatomer protein complex, subunit epsilon; coatomer subunit epsilon; epsilon coat protein; Epsilon-coat protein; epsilon-COP; FLJ13241 COPE epsilon-COP COPI coat complex subunit epsilon coatomer subunit epsilon|coatomer epsilon subunit|coatomer protein complex subunit epsilon|epsilon coat protein

For more info on COPE, check out the COPE Infographic

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In this infographic, you will see the following information for COPE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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