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Product Info Summary
| SKU: | M00714-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human Emerin DyLight® 488 conjugated EMD Antibody(monoclonal, 5A10)
SKU/Catalog Number
M00714-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Emerin DyLight® 488 conjugated EMD Antibody (monoclonal, 5A10) catalog # M00714-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Emerin DyLight® 488 conjugated EMD Antibody(monoclonal, 5A10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00714-Dyl488)
Host
Mouse
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Monoclonal
Clone Number
5A10
Isotype
Mouse IgG1
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Emerin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00714-Dyl488 is reactive to EMD in Human
Applications
M00714-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
28.994kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For EMD (Source: Uniprot.org, NCBI)
Gene Name
EMD
Full Name
Emerin
Weight
28.994kDa
Alternative Names
emerin; Emery-Dreifuss muscular dystrophy; LEM domain containing 5; STAEDMDLEMD5 EMD EDMD, LEMD5, STA emerin emerin|LEM domain containing 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EMD, check out the EMD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EMD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Emerin DyLight® 488 conjugated EMD Antibody(monoclonal, 5A10) (M00714-Dyl488)
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No publications found for M00714-Dyl488
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human Emerin DyLight® 488 conjugated EMD Antibody(monoclonal, 5A10)
Question
We are currently using anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
M. Parker
Verified customer
Asked: 2020-04-28
Answer
The anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) (M00714-Dyl488) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-28
Question
I see that the anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-13
Question
Would M00714-Dyl488 anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Johnson
Verified customer
Asked: 2019-10-15
Answer
You can see on the product datasheet, M00714-Dyl488 anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-15
Question
Is there a BSA free version of anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488 available?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-22
Question
I was wanting to use your anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) for Flow Cytometry for human placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
You can see on the product datasheet, M00714-Dyl488 anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-12
Question
My colleagues were well pleased with the WB result of your anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10). However we have seen positive staining in cervix carcinoma embryonic kidney nucleus inner membrane using this antibody. Is that expected? Could you tell me where is EMD supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
According to literature, cervix carcinoma embryonic kidney does express EMD. Generally EMD expresses in nucleus inner membrane. Regarding which tissues have EMD expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Embryonic kidney, Pubmed ID: 9673989
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Teratocarcinoma, Pubmed ID: 7894480
Boster Scientific Support
Answered: 2018-09-06
Question
We need to test anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488 on human placenta for research purposes, then I may be interested in using anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
The products we sell, including anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-23
Question
We have observed staining in human teratocarcinoma. Do you have any suggestions? Is anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) supposed to stain teratocarcinoma positively?
G. Walker
Verified customer
Asked: 2018-06-19
Answer
From what I have seen in literature teratocarcinoma does express EMD. From what I have seen in Uniprot.org, EMD is expressed in esophagogastric junction muscularis propria, teratocarcinoma, placenta, platelet, cervix carcinoma embryonic kidney, leukemic t-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have EMD expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Embryonic kidney, Pubmed ID: 9673989
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Teratocarcinoma, Pubmed ID: 7894480
Boster Scientific Support
Answered: 2018-06-19
Question
Does anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488 work for Flow Cytometry with placenta?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
According to the expression profile of placenta, EMD is highly expressed in placenta. So, it is likely that anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) M00714-Dyl488 will work for Flow Cytometry with placenta.
Boster Scientific Support
Answered: 2017-08-01
Question
Is a blocking peptide available for product anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) (M00714-Dyl488)?
A. Roberts
Verified customer
Asked: 2017-02-06
Answer
We do provide the blocking peptide for product anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) (M00714-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-02-06
Question
Can you help my question with product M00714-Dyl488, anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Singh
Verified customer
Asked: 2015-07-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00714-Dyl488 anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-08
Question
Is this M00714-Dyl488 anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) reactive to the isotypes of EMD?
S. Patel
Verified customer
Asked: 2015-04-14
Answer
The immunogen of M00714-Dyl488 anti-Human Emerin DyLight® 488 conjugated antibody(monoclonal, 5A10) is A synthetic peptide corresponding to a sequence at the N-terminus of human Emerin (1-48aa MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRL), different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-04-14
