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Product Info Summary
| SKU: | M05183-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123
SKU/Catalog Number
M05183-1
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 (Catalog # M05183-1). Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05183-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM123
Isotype
Rabbit IgG
Immunogen
Human IgD
Cross-reactivity
This antibody reacts to human IgD. No cross-reactivity with human IgG, IgM, IgA, or IgE.
Reactive Species
M05183-1 is reactive to IGHD in Human
Calculated molecular weight
42.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05183-1 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 25ng/well – 200ng/well (for Capture)
0.01ug/mL – 0.1ug/mL (for Detection)
Validation Images & Assay Conditions
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IHC result
Immunohistochemistry of human lymphoid tissue using anti-human IgD antibody RM123
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ELISA result showing specificity
Sandwich ELISA using RM123 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM123 reacted to the IgD from human plasma and the IgD from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM123. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 (M05183-1)
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1 Customer Q&As for Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123
Question
We are currently using anti-Human IgD Rabbit Monoclonal antibody, Clone#RM123 M05183-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
H. Jones
Verified customer
Asked: 2016-08-05
Answer
The anti-Human IgD Rabbit Monoclonal antibody, Clone#RM123 (M05183-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-05
