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Product Info Summary
| SKU: | M05183 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IGHD Monoclonal Antibody
SKU/Catalog Number
M05183
BM5670 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IGHD Monoclonal Antibody catalog # M05183. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGHD Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05183)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFBO-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IGHD There are two named isoforms of IGHD produced by alternative splicing, a secreted form and a single pass type I membrane protein.
Reactive Species
M05183 is reactive to IGHD in Human
Observed Molecular Weight
84 kDa
Calculated molecular weight
42.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05183 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: Humantonsil cell
Validation Images & Assay Conditions
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Western blot analysis of IGHD expression in Humantonsil cell lysate.
Specific Publications For Anti-IGHD Monoclonal Antibody (M05183)
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Customer Q&As
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4 Customer Q&As for Anti-IGHD Monoclonal Antibody
Question
Does anti-IGHD Monoclonal antibody M05183 work on zebrafish WB with b-cell?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Our lab technicians have not validated anti-IGHD Monoclonal antibody M05183 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IGHD Monoclonal antibody M05183 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish b-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-19
Question
I was wanting to use your anti-IGHD Monoclonal antibody for WB for human trachea on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human trachea identification?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
You can see on the product datasheet, M05183 anti-IGHD Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human trachea in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-08
Question
I see that the anti-IGHD Monoclonal antibody M05183 works with WB, what is the protocol used to produce the result images on the product page?
A. Yang
Verified customer
Asked: 2017-02-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-02-09
Question
My question regarding product M05183, anti-IGHD Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Mitchell
Verified customer
Asked: 2015-09-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05183 anti-IGHD Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-09-15
