Click image to see more details
Product Info Summary
Customers Who Bought This Also Bought
Anti-IGHD Monoclonal Antibody
Boster Bio Anti-IGHD Monoclonal Antibody catalog # M05183. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGHD Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05183)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human IGHD There are two named isoforms of IGHD produced by alternative splicing, a secreted form and a single pass type I membrane protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M05183 is reactive to IGHD in Human
M05183 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 IHC 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IGHD using anti-IGHD antibody in Humantonsil cell lysate. (M05183).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGHD antigen affinity purified polyclonal antibody (Catalog # M05183) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Gene/Protein Information For IGHD (Source: Uniprot.Org, NCBI)
Immunoglobulin heavy constant delta
IgD; Igh-5; Ighd; Immunoglobulin D; Immunoglobulin Delta IGHD immunoglobulin heavy constant delta constant region of heavy chain of IgD*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IGHD, check out the IGHD Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGHD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M05183
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Anti-IGHD Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-IGHD Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question