Product Info Summary
SKU: | B04429 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody
View all IL-17E/IL-25 Antibodies
SKU/Catalog Number
B04429
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody (Catalog # B04429). Tested in Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # B04429)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 11K8B1 IgG1
Isotype
IgG1
Immunogen
Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
B04429 is reactive to IL25 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
20330 MW
Background of IL-17E/IL-25
Anti-IL17E recognizes IL-17E (also known as IL25). IL-17E is produced and secreted by intra-epithelial lymphocytes, lung epithelial cells, alveolar macrophages, eosinophils, basophils, NKT cells, mast cells and cells of the gastro-intestinal tract and uterus. IL-17E stimulates the production of Th2-type cytokines. IL-17E limits Th17 cell development by inducing the expression of IL-13 by dendritic cells or by inhibiting IL-23 production by macrophages. Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. A number of cytokines, including IL-17E, have been implicated as oncogenes, reflecting the importance of these proteins in cell growth and survival. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and pro-inflammatory pathologies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
B04429 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
Flow Cytometry: 1µg/mL
Fluorochrome Protein Value: 10-20
WB: 0.5µg/mL
Validation Images & Assay Conditions
Click image to see more details
Flow cytometry analysis of PC3 cells expressing IL-17E using 1 μg of mouse anti-IL-17E monoclonal antibody (Catalog # B04429) (red line) or control (green line). Anti-mouse IgG PE conjugated secondary antibodywas used to detect the primary antibody.
Click image to see more details
Western blot analysis of IL-17E expression in full length recombinant protein. IL-17E was detected using mouse anti-IL-17E antibody monoclonal antibody (Catalog # B04429) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Protein Target Info & Infographic
Gene/Protein Information For IL25 (Source: Uniprot.org, NCBI)
Gene Name
IL25
Full Name
Interleukin-25
Weight
20330 MW
Superfamily
IL-17 family
Alternative Names
IL-17E, IL-25, Interleukin 25, IL-25, Interleukin 17E, IL-17E IL25 IL17E interleukin 25 interleukin-25|interleukin-17E
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL25, check out the IL25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody (B04429)
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