Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody

CD81 antibody

Boster Bio Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody catalog # A01281-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A01281-Dyl488
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

Product Name

Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody

View all CD81 Antibodies

SKU/Catalog Number

A01281-Dyl488

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody catalog # A01281-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01281-Dyl488)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human TAPA1 recombinant protein (Position: F113-K201).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01281-Dyl488 is reactive to CD81 in Human

Applications

A01281-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

25.809kDa

Background of CD81

CD81 molecule, also known as CD81 (Cluster of Differentiation 81), is a protein which in humans is encoded by the CD81 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For CD81 (Source: Uniprot.org, NCBI)

Gene Name

CD81

Full Name

CD81 antigen

Weight

25.809kDa

Superfamily

tetraspanin (TM4SF) family

Alternative Names

CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81; CVID6; TAPA1; TAPA-1; Target of the antiproliferative 1; Tetraspanin-28; TSPAN28; Tspan-28; tspan-28,26 kDa cell surface protein TAPA-1; TSPAN28S5.7 CD81 CVID6, S5.7, TAPA1, TSPAN28 CD81 molecule CD81 antigen|26 kDa cell surface protein TAPA-1|CD81 antigen (target of antiproliferative antibody 1)|tetraspanin-28|tspan-28

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD81, check out the CD81 Infographic

CD81 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD81: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody

Question

We are currently using anti-Human TAPA1 DyLight® 488 conjugated antibody A01281-Dyl488 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

The anti-Human TAPA1 DyLight® 488 conjugated antibody (A01281-Dyl488) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-09

Question

We have been able to see staining in human myometrium. Are there any suggestions? Is anti-Human TAPA1 DyLight® 488 conjugated antibody supposed to stain myometrium positively?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

From what I have seen in literature myometrium does express CD81. From what I have seen in Uniprot.org, CD81 is expressed in myometrium, brain, among other tissues. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-01-03

Question

Is this A01281-Dyl488 anti-Human TAPA1 DyLight® 488 conjugated antibody reactive to the isotypes of CD81?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

The immunogen of A01281-Dyl488 anti-Human TAPA1 DyLight® 488 conjugated antibody is E. coli-derived human TAPA1 recombinant protein (Position: F113-K201). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-25

Question

We were satisfied with the WB result of your anti-Human TAPA1 DyLight® 488 conjugated antibody. However we have been able to see positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is CD81 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-01-25

Answer

From literature, brain does express CD81. Generally CD81 expresses in cell membrane. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-01-25

Question

We need to test anti-Human TAPA1 DyLight® 488 conjugated antibody A01281-Dyl488 on human myometrium for research purposes, then I may be interested in using anti-Human TAPA1 DyLight® 488 conjugated antibody A01281-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

L. Moore

Verified customer

Asked: 2018-03-13

Answer

The products we sell, including anti-Human TAPA1 DyLight® 488 conjugated antibody A01281-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-03-13

Question

I was wanting to use your anti-Human TAPA1 DyLight® 488 conjugated antibody for Flow Cytometry for human myometrium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human myometrium identification?

J. Krishna

Verified customer

Asked: 2017-08-25

Answer

As indicated on the product datasheet, A01281-Dyl488 anti-Human TAPA1 DyLight® 488 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human myometrium in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-25

Order DetailsPrice
A01281-Dyl488

100μg

$515
A01281-Dyl488-carrier-free

Carrier Free

$515

Free Secondary Antibody

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A01281-Dyl488
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

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