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Product Info Summary
| SKU: | M01025-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-DC-SIGN CD209 Monoclonal Antibody [5D7]
SKU/Catalog Number
M01025-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DC-SIGN CD209 Monoclonal Antibody [5D7] (Catalog # M01025-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human.
Storage & Handling
DC-SIGN monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-DC-SIGN CD209 Monoclonal Antibody [5D7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01025-1)
Host
Mouse
Contents
DC-SIGN Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 5D7
Isotype
IgG1
Immunogen
A recombinant His-tagged protein fragment corresponding to the extracellular region of human DC-SIGN.
Reactive Species
M01025-1 is reactive to CD209 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
45.8 kDa
Background of CD209
Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01025-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DC-SIGN antibody can be used for detection of DC-SIGN in Western Blot at 1 μg/mL and in immunohistochemistry at 5 - 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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WB Validation of DC-SIGN in Human Lymph Node
Loading: 10 μg of lysate per lane.
Antibodies: DC-SIGN M01025-1, 1ug/ml, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Lane 1: 1 ug/mL
Lane 2: 2 ug/mL
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Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 5 μg/mL.
Specific Publications For Anti-DC-SIGN CD209 Monoclonal Antibody [5D7] (M01025-1)
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