Anti-Checkpoint protein HUS1B Hus1B Antibody

Product Name

Anti-Checkpoint protein HUS1B Hus1B Antibody

View all HUS1B Antibodies

SKU/Catalog Number

A13393

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Checkpoint protein HUS1B Hus1B Antibody (Catalog # A13393). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Checkpoint protein HUS1B Hus1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13393)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human Hus1B protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A13393 is reactive to HUS1B in Human

Applications

A13393 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

30.982kDa

Background of HUS1B

The protein encoded by the Hus1B gene is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the checkpoint protein RAD1 but not with RAD9. Over expression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to HUS1. Hus1B is expressed strongly in testis, less in spleen, thymus, prostate, colon and leukocytes. Anti-HUSB1 Antibody is useful for researchers interested in DNA damage and repair.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IP: 1:100
WB: 1:500 - 1:5,000

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of Hus1B expression in HELA cell lysate (arrowhead). Hus1B at 36KD was detected using rabbit anti-Hus1B Antigen Affinity purified polyclonal antibody (Catalog # A13393). The staining pattern in 293 cells is less clear, showing a predominant band at 39 kDa. Personal Communication, A-Lien Lu-Chang, U. Maryland, Baltimore, MD

Gene/Protein Information For HUS1B (Source: Uniprot.org, NCBI)

Alternative Names

checkpoint protein HUS1B; hHUS1B; HUS1 (S. pombe) checkpoint homolog b; HUS1 checkpoint homolog b (S. pombe); MGC126746; MGC126748; RP11-532F6.1 HUS1B HUS1 checkpoint clamp component B checkpoint protein HUS1B|HUS1 checkpoint homolog b|hHUS1B

For more info on HUS1B, check out the HUS1B Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HUS1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the HUS1B Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis