|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-HYAL2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Hyaluronidase-2(HYAL2) detection. Tested with WB in Human;Mouse;Rat. .|
|Cite This Product||Anti-HYAL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1817)|
|Replacement Item||This antibody may replace the following items: sc-49205|sc-99022 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human HYAL2(142-157aa VRNWQDKDVYRRLSRQ), different from the related mouse and rat sequences by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||53860 MW|
|Protein Function||Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. .|
|Tissue Specificity||Widely expressed. No expression detected in adult brain. .|
|Sequence Similarities||Belongs to the glycosyl hydrolase 56 family.|
|Subcellular Localization||Cell membrane ; Lipid-anchor, GPI-anchor .|
|Alternative Names||Hyaluronidase-2;Hyal-2;18.104.22.168;Hyaluronoglucosaminidase-2;Lung carcinoma protein 2;LuCa-2;HYAL2;LUCA2;|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|other enzymes| cancer|invasion/microenvironment||
Background for Hyaluronidase-2(Hyal-2)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HYAL2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-HYAL2 Antibody Images
Click the images to enlarge.
All lanes: Anti HYAL2 (PA1817) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 54KD
Observed bind size: 54KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,