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Anti-ICAM1 Antibody Picoband™
Boster Bio Anti-ICAM1 Antibody Picoband™ catalog # PB9004. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ICAM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9004)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
NSO-derived human ICAM1 recombinant protein (Position: Q28-E480). Human ICAM1 shares 55% and 53% amino acid (aa) sequences identity with mouse and rat ICAM1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9004 is reactive to ICAM1 in Human
PB9004 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9004).
ICAM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ICAM1 Antibody (PB9004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9004).
ICAM1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ICAM1 Antibody (PB9004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Western blot analysis of ICAM1 using anti-ICAM1 antibody (PB9004).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # PB9004) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 100 kDa. The expected band size for ICAM1 is at 58 kDa.
Gene/Protein Information For ICAM1 (Source: Uniprot.Org, NCBI)
Intercellular adhesion molecule 1
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ICAM1, check out the ICAM1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ICAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PB9004 has been cited in 37 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells
Trimethylamine N-oxide in atherogenesis: impairing endothelial self-repair capacity and enhancing monocyte adhesion
The Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress
Effect of Puerarin on Expression of ICAM-1 and TNF-? in Kidneys of Diabetic Rats
Retinoic Acid Promotes Interleukin-4 Plasmid-Dimethylsulfoxide Topical Transdermal Delivery for Treatment of Psoriasis
Administration of pigment epithelium-derived factor delivered by adeno-associated virus inhibits blood?retinal barrier breakdown in diabetic rats
Effect of nuclear factor kappa B on intercellular adhesion molecule-1 expression and neutrophil infiltration in lung injury induced by intestinal ischemia/reperfusion in rats
Influence of platelet activating factor on expression of adhesion molecules in experimental pancreatitis
Octreotide Protects the Mouse Retina against Ischemic Reperfusion Injury through Regulation of Antioxidation and Activation of NF-?B
Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke
Ma, G., Pan, B., Ren, S., Guo, C., Guo, Y., Wei, L.,…, & Chen, B. (2017). 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell. Lipids in Health and Disease, 16(1), 137. doi: 10.1186/s12944-017-0518-2
Zhu W, Lv Q, Chen H, Wang Z, Zhong Q. J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):441-5. Doi: 10.1007/S11596-011-0470-8. Epub 2011 Aug 7. Protective Effect And Mechanism Of Sodium Tanshinone Ii A Sulfonate On Microcirculatory Disturbance...
Wang Zz, Zhao Wj, Zhang Xs, Tian Xf, Wang Yz, Zhang F, Yuan Jc, Han Gz, Liu Kx, Yao Jh. World J Gastroenterol. 2007 Jan 28;13(4):564-71. Protection Of Veratrum Nigrum L. Var. Ussuriense Nakai Alkaloids Against Ischemia-Reperfusion Injury Of The Ra...
Zhang Y, Li J, Liu Cy, Zhou Xk, Qiu J, Zhang Yb, Huang Ny, Li Y, Chen Xj, Li Xl, Wang Ys, Yang Hs, Chen Xc, Kan B, Mao Yq, Deng Hx, Yang L, Wen Yj, Zhao X, Wei Yq. Dermatology. 2010 Aug;221(1):84-92. Doi: 10.1159/000314154. Epub 2010 Jun 18. A Nov...
Tian Xf, Yao Jh, Zhang Xs, Zheng Ss, Guo Xh, Wang Lm, Wang Zz, Liu Kx. Surg Today. 2010 Sep;40(9):858-65. Doi: 10.1007/S00595-009-4170-Y. Epub 2010 Aug 26. Protective Effect Of Carnosol On Lung Injury Induced By Intestinal Ischemia/Reperfusion.
Cheng Y, Zhang Y, Song H, Feng J. Neural Regen Res. 2012 Jun 15;7(17):1312-7. Doi: 10.3969/J.Issn.1673-5374.2012.17.005. Intercellular Adhesion Molecule-1 Expression In The Hippocampal Ca1 Region Of Hyperlipidemic Rats With Chronic Cerebral Ischemia.
Liu D, Zeng Bx, Zhang Sh, Yao Sl. Inflamm Res. 2005 Nov;54(11):464-70. Rosiglitazone, An Agonist Of Peroxisome Proliferator-Activated Receptor Gamma, Reduces Pulmonary Inflammatory Response In A Rat Model Of Endotoxemia.
Hang Ch, Shi Jx, Li Js, Li Wq, Yin Hx. World J Gastroenterol. 2005 Feb 28;11(8):1149-54. Up-Regulation Of Intestinal Nuclear Factor Kappa B And Intercellular Adhesion Molecule-1 Following Traumatic Brain Injury In Rats.
Hang Ch, Shi Jx, Tian J, Li Js, Wu W, Yin Hx. Brain Res. 2004 Nov 5;1026(1):23-32. Effect Of Systemic Lps Injection On Cortical Nf-Kappab Activity And Inflammatory Response Following Traumatic Brain Injury In Rats.
Wang J, Qiao L, Li Y, Yang G. Exp Mol Med. 2008 Dec 31;40(6):686-98. Ginsenoside Rb1 Attenuates Intestinal Ischemia-Reperfusion- Induced Liver Injury By Inhibiting Nf-Kappab Activation.
Wu J, Yang T, Wang C, Liu Q, Yao J, Sun H, Kaku T, Liu Kx. Biol Pharm Bull. 2008 Nov;31(11):2040-4. Laennec Protects Murine From Concanavalin A-Induced Liver Injury Through Inhibition Of Inflammatory Reactions And Hepatocyte Apoptosis.
Yao Jh, Zhang Xs, Zheng Ss, Li Yh, Wang Lm, Wang Zz, Chu L, Hu Xw, Liu Kx, Tian Xf. World J Gastroenterol. 2009 Jul 14;15(26):3240-5. Prophylaxis With Carnosol Attenuates Liver Injury Induced By Intestinal Ischemia/Reperfusion.
Xu Y, Feng L, Wang S, Zhu Q, Zheng Z, Xiang P, He B, Tang D. J Ethnopharmacol. 2011 Sep 1;137(1):359-70. Doi: 10.1016/J.Jep.2011.05.041. Epub 2011 Jun 6. Calycosin Protects Huvecs From Advanced Glycation End Products-Induced Macrophage Infiltration.
Wang W, Zhao F, Fang Y, Li X, Shen L, Cao T, Zhu H. Crit Care. 2013 Mar 11;17(2):R44. Doi: 10.1186/Cc12558. Glycyrrhizin Protects Against Porcine Endotoxemia Through Modulation Of Systemic Inflammatory Response.
Tu Ct, Han B, Liu Hc, Zhang Sc. Mol Cell Biochem. 2011 Dec;358(1-2):53-60. Doi: 10.1007/S11010-011-0920-4. Epub 2011 Jun 22. Curcumin Protects Mice Against Concanavalin A-Induced Hepatitis By Inhibiting Intrahepatic Intercellular Adhesion Molecule...
Ning C, Liu L, Lv G, Yang Y, Zhang Y, Yu R, Wang Y, Zhu J. Lipids Health Dis. 2015 Apr 18;14:31. Doi: 10.1186/S12944-015-0030-5. Lipid Metabolism And Inflammation Modulated By Vitamin D In Liver Of Diabetic Rats.
Wang Yq, Shen Y, Li F, Wang Ch, Zhang W. Cell Biochem Biophys. 2013;67(3):997-1004. Doi: 10.1007/S12013-013-9595-7. 2,3,4',5-Tetrahydroxystilbene-2-O-??-D-Glucoside Suppresses Expression Of Adhesion Molecules In Aortic Wall Of Dietary Atherosclero...
Li Y, Chen Y, Zhang J, Zhu Jf, Liu Zj, Liang Sy, Sun K, Liao Wy, Gong Jp. Am J Surg. 2010 Jan;199(1):35-42. Doi: 10.1016/J.Amjsurg.2008.11.039. Protective Effect Of Glutamine-Enriched Early Enteral Nutrition On Intestinal Mucosal Barrier Injury Af...
Ji X, Gao J, Cai X, Lu W, Hu C, Wang Z, Cheng X, Gu Z, Wan G, Zhang S, Cao P. J Ethnopharmacol. 2011 Apr 12;134(3):624-9. Doi: 10.1016/J.Jep.2011.01.003. Epub 2011 Jan 8. Immunological Regulation Of Chinese Herb Guizhi Fuling Capsule On Rat Endome...
Cui Wy, Tian Ay, Bai T. Clin Exp Pharmacol Physiol. 2011 Nov;38(11):747-54. Doi: 10.1111/J.1440-1681.2011.05584.X. Protective Effects Of Propofol On Endotoxemia-Induced Acute Kidney Injury In Rats.
Huang Kf, Huang Xp, Xiao Gq, Yang Hy, Lin Js, Diao Y. Biomed Pharmacother. 2014 May;68(4):455-61. Doi: 10.1016/J.Biopha.2014.03.005. Epub 2014 Mar 18. Kallistatin, A Novel Anti-Angiogenesis Agent, Inhibits Angiogenesis Via Inhibition Of The Nf-??b...
Fan Z, Jing H, Yao J, Li Y, Hu X, Shao H, Shen G, Pan J, Luo F, Tian X. Oxid Med Cell Longev. 2014;2014:191624. Doi: 10.1155/2014/191624. Epub 2014 Aug 20. The Protective Effects Of Curcumin On Experimental Acute Liver Lesion Induced By Intestinal...
Fan Z, Yao J, Li Y, Hu X, Shao H, Tian X. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3451-9. Ecollection 2015. Anti-Inflammatory And Antioxidant Effects Of Curcumin On Acute Lung Injury In A Rodent Model Of Intestinal Ischemia Reperfusion By Inhibitin...
Zhang Yf, Yang X, Zhang Yj, Sun Yl, Zou Xl, Kong Qy, Dong Xq, Ye Xq, Yu Xq. Am J Nephrol. 2006;26(6):602-11. Epub 2006 Dec 20. Peroxisome Proliferator-Activated Receptor-Gamma Is Expressed By Rat Peritoneal Mesothelial Cells: Its Potential Role In...
Xiang M, Qian Zy, Zhou Ch, Liu J, Li Wn. J Ethnopharmacol. 2006 Aug 11;107(1):25-31. Epub 2006 Mar 10. Crocetin Inhibits Leukocyte Adherence To Vascular Endothelial Cells Induced By Ages.
Tian Xx, Wang Bl, Cao Yz, Zhong Yx, Tu Yy, Xiao Jb, He Qf, Zhai Ln. Chin J Integr Med. 2015 Mar;21(3):229-33. Doi: 10.1007/S11655-013-1513-8. Epub 2013 Oct 19. Comparison Of Protective Effects Of Safflor Injection And Extract Of Ginkgo Biloba On L...
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...
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