Anti-ID4 Antibody

ID4 antibody

Boster Bio Anti-ID4 Antibody (Catalog # A03975). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A03975
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-ID4 Antibody

View all ID4 Antibodies

SKU/Catalog Number

A03975

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ID4 Antibody (Catalog # A03975). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ID4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03975)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ID4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03975 is reactive to ID4 in Human, Mouse, Rat

Applications

A03975 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

16.622kDa

Background of ID4

ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

Rigolet M., DNA Res. 5:309-313(1998).
Pagliuca A., Genomics 27:200-203(1995).
Mungall A.J., Nature 425:805-811(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For ID4 (Source: Uniprot.org, NCBI)

Gene Name

ID4

Full Name

DNA-binding protein inhibitor ID-4

Weight

16.622kDa

Alternative Names

bHLHb27BHLHB27; Class B basic helix-loop-helix protein 27; DNA-binding protein inhibitor ID-4; IDB4; Inhibitor of DNA binding 4; inhibitor of DNA binding 4, dominant negative helix-loop-helix protein ID4 IDB4, bHLHb27 inhibitor of DNA binding 4, HLH protein DNA-binding protein inhibitor ID-4|class B basic helix-loop-helix protein 27|inhibitor of DNA binding 4, dominant negative helix-loop-helix protein|inhibitor of differentiation 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ID4, check out the ID4 Infographic

ID4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ID4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A03975 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells

PAX7 promotes CD49f‐positive dairy goat spermatogonial stem cells self‐renewal

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5 Customer Q&As for Anti-ID4 Antibody

Question

See attached the WB image, lot number and protocol we used for abdominal adipose tissue using anti-ID4 antibody A03975. Please let me know if you require anything else.

T. Edwards

Verified customer

Asked: 2018-09-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-27

Question

We are currently using anti-ID4 antibody A03975 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-20

Answer

The anti-ID4 antibody (A03975) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-20

Question

Does A03975 anti-ID4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-12-21

Answer

It shows on the product datasheet, A03975 anti-ID4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-12-21

Question

My question regarding product A03975, anti-ID4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-09-07

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03975 anti-ID4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-09-07

Question

I see that the anti-ID4 antibody A03975 works with WB, what is the protocol used to produce the result images on the product page?

K. Roberts

Verified customer

Asked: 2013-03-01

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-03-01

Order DetailsPrice
A03975-100uL

100uL

$360

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