Anti-IDO1/Ido Monoclonal Antibody

Product Name

Anti-IDO1/Ido Monoclonal Antibody

View all Indoleamine 2,3-dioxygenase/IDO Antibodies

SKU/Catalog Number

M01705-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-IDO1/Ido Monoclonal Antibody (Catalog # M01705-1). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-IDO1/Ido Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01705-1)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 10.1 IgG3

Isotype

IgG3

Immunogen

Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01705-1 is reactive to IDO1 in Human, Mouse

Applications

M01705-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

45.326kDa

Background of Indoleamine 2,3-dioxygenase/IDO

Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5000-1:50000
IHC: 1:100-1:500
IF Microscopy: 1:50-1:100
IP: 10-100 µL
WB: 1:500-1:1500

Validation Images & Assay Conditions

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Western blot analysis of IDO1 expression in untreated HeLa cells (lane 1) and IFN-r treated HeLa cells (lane 2). IDO1 at 41-42KD was detected using mouse anti-IDO1 Antigen Affinity purified monoclonal antibody (Catalog # M01705-1) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

Gene/Protein Information For IDO1 (Source: Uniprot.org, NCBI)

Alternative Names

3dioxygenase; EC 1.13.11.52; IDO; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; Indoleamine 2; indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase IDO1 IDO, IDO-1, INDO indoleamine 2,3-dioxygenase 1 indoleamine 2,3-dioxygenase 1|indolamine 2,3 dioxygenase|indole 2,3-dioxygenase|indoleamine-pyrrole 2,3-dioxygenase

For more info on IDO1, check out the IDO1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IDO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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