Product Info Summary
SKU: | PA1611 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody
View all Indoleamine 2,3-dioxygenase/IDO Antibodies
SKU/Catalog Number
PA1611
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody catalog # PA1611. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1611)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IDO1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1611 is reactive to IDO1 in Human
Applications
PA1611 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
45.326kDa
Background of Indoleamine 2,3-dioxygenase/IDO
IDO1 (INDOLEAMINE 2,3-DIOXYGENASE), INDO or IDO, is an immunomodulatory enzyme produced by some alternatively activated macrophages and other immunoregulatory cells. This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. By fluorescence in situ hybridization, the assignment is narrowed to chromosome 8p12-p11. INDO Interferon-gamma has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase. During inflammation, IDO is upregulated in dendritic cells and phagocytes by proinflammatory stimuli, most notably IFNG, and the enzyme then uses superoxide as a 'cofactor' for oxidative cleavage of the indole ring of tryptophan, yielding an intermediate that deformylates to L-kynurenine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-IDO1 antibody, PA1611, Western blotting
All lanes: Anti IDO1 (PA1611) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: 293T Whole Cell Lysate at 40ug
Lane 7: A431 Whole Cell Lysate at 40ug
Lane 8: HELA Whole Cell Lysate at 40ug
Lane 9: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
Protein Target Info & Infographic
Gene/Protein Information For IDO1 (Source: Uniprot.org, NCBI)
Gene Name
IDO1
Full Name
Indoleamine 2,3-dioxygenase 1
Weight
45.326kDa
Superfamily
indoleamine 2,3-dioxygenase family
Alternative Names
3dioxygenase; EC 1.13.11.52; IDO; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; Indoleamine 2; indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase IDO1 IDO, IDO-1, INDO indoleamine 2,3-dioxygenase 1 indoleamine 2,3-dioxygenase 1|indolamine 2,3 dioxygenase|indole 2,3-dioxygenase|indoleamine-pyrrole 2,3-dioxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IDO1, check out the IDO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IDO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody (PA1611)
Hello CJ!
PA1611 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of indoleamine 2, 3-dioxygenase in nasopharyngeal carcinoma impairs the cytolytic function of peripheral blood lymphocytes
Umbilical Cord Tissue-Derived Mesenchymal Stem Cells Induce T Lymphocyte Apoptosis and Cell Cycle Arrest by Expression of Indoleamine 2, 3-Dioxygenase
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody
Question
I see that the anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody PA1611 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-27
Question
My lab would like to test anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody PA1611 on human placenta for research purposes, then I may be interested in using anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody PA1611 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The products we sell, including anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody PA1611, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-18
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody PA1611. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
Do you have a BSA free version of anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody PA1611 available?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody PA1611 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-04
Question
Does PA1611 anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
It shows on the product datasheet, PA1611 anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-02
Question
Will anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody PA1611 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
According to the expression profile of placenta, IDO1 is highly expressed in placenta. So, it is likely that anti-Indoleamine 2, 3-dioxygenase/IDO1 antibody PA1611 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-01-07