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Product Info Summary
| SKU: | M00135-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-IF4E Antibody
SKU/Catalog Number
M00135-2
Size
80 µl
Description
Boster Bio Anti-IF4E Antibody (Catalog # M00135-2). Tested in WB, IF application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-IF4E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00135-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1504CT774.14.55
Isotype
IgG1,Κ
Immunogen
This IF4E antibody is generated from a mouse immunized with a recombinant protein of human IF4E.
Reactive Species
M00135-2 is reactive to EIF4E in Human, Mouse, Rat
Calculated molecular weight
25.1 kDa
Background of EIF4E
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00135-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:4000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling IF4E with M00135-2 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-IF4E Antibody at 1:4000 dilution
Lane 1: Hela whole cell lysates
Lane 2: MCF-7 whole cell lysates
Lane 3: K562 whole cell lysates
Lane 4: NIH/3T3 whole cell lysates
Lane 5: PC-12 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-IF4E Antibody (M00135-2)
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