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Product Info Summary
| SKU: | A02652 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IFIT1 Antibody
SKU/Catalog Number
A02652
Size
100ul
Form
Liquid
Description
Boster Bio Anti-IFIT1 Antibody (Catalog# A02652). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IFIT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02652)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human IFIT1. Immunogen sequence location: 41-90
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02652 is reactive to IFIT1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
55.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02652 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from LOVO cells, using IFIT1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-IFIT1 Antibody (A02652)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-IFIT1 Antibody
Question
My question regarding product A02652, anti-IFIT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02652 anti-IFIT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-12
Question
We are currently using anti-IFIT1 antibody A02652 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
J. Brown
Verified customer
Asked: 2020-01-14
Answer
The anti-IFIT1 antibody (A02652) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-14
Question
Does anti-IFIT1 antibody A02652 work for WB with prostate?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
According to the expression profile of prostate, IFIT1 is highly expressed in prostate. So, it is likely that anti-IFIT1 antibody A02652 will work for WB with prostate.
Boster Scientific Support
Answered: 2019-06-17
Question
My question regards to test anti-IFIT1 antibody A02652 on human prostate for research purposes, then I may be interested in using anti-IFIT1 antibody A02652 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
The products we sell, including anti-IFIT1 antibody A02652, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-27
Question
Would anti-IFIT1 antibody A02652 work on canine WB with epithelium of bronchus?
A. Roberts
Verified customer
Asked: 2017-02-06
Answer
Our lab technicians have not tested anti-IFIT1 antibody A02652 on canine. You can run a BLAST between canine and the immunogen sequence of anti-IFIT1 antibody A02652 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine epithelium of bronchus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-06
