Anti-IFIT1 Antibody

IFIT1 antibody

Boster Bio Anti-IFIT1 Antibody (Catalog# A02652). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse. Cited in 1 publication(s).

Product Info Summary

SKU: A02652
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB, IF (paraffin)

Product Name

Anti-IFIT1 Antibody

View all IFIT1 Antibodies

SKU/Catalog Number

A02652

Size

100ul

Form

Liquid

Description

Boster Bio Anti-IFIT1 Antibody (Catalog# A02652). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IFIT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02652)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human IFIT1. Immunogen sequence location: 41-90

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A02652 is reactive to IFIT1 in Human, Mouse, Rat

Applications

A02652 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

55.36kDa

Background of IFIT1

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For IFIT1 (Source: Uniprot.org, NCBI)

Gene Name

IFIT1

Full Name

Interferon-induced protein with tetratricopeptide repeats 1

Weight

55.36kDa

Superfamily

IFIT family

Alternative Names

G10P1C56; GARG-16; IFI56IFI-56; IFI-56K; IFIT-1; IFNAI1ISG56; Interferon, alpha-inducible protein (MW 56kD); Interferon-induced 56 kDa protein; interferon-induced protein 56; interferon-induced protein with tetratricopeptide repeats 1; p56; RNM561 IFIT1 C56, G10P1, IFI-56, IFI-56K, IFI56, IFIT-1, IFNAI1, ISG56, P56, RNM561 interferon induced protein with tetratricopeptide repeats 1 interferon-induced protein with tetratricopeptide repeats 1|interferon, alpha-inducible protein (MW 56kD)|interferon-induced 56 kDa protein|interferon-inducible mRNA 561

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IFIT1, check out the IFIT1 Infographic

IFIT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IFIT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A02652 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1

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5 Customer Q&As for Anti-IFIT1 Antibody

Question

My question regarding product A02652, anti-IFIT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02652 anti-IFIT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-12

Question

We are currently using anti-IFIT1 antibody A02652 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

J. Brown

Verified customer

Asked: 2020-01-14

Answer

The anti-IFIT1 antibody (A02652) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-14

Question

Does anti-IFIT1 antibody A02652 work for WB with prostate?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

According to the expression profile of prostate, IFIT1 is highly expressed in prostate. So, it is likely that anti-IFIT1 antibody A02652 will work for WB with prostate.

Boster Scientific Support

Answered: 2019-06-17

Question

My question regards to test anti-IFIT1 antibody A02652 on human prostate for research purposes, then I may be interested in using anti-IFIT1 antibody A02652 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-10-27

Answer

The products we sell, including anti-IFIT1 antibody A02652, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-10-27

Question

Would anti-IFIT1 antibody A02652 work on canine WB with epithelium of bronchus?

A. Roberts

Verified customer

Asked: 2017-02-06

Answer

Our lab technicians have not tested anti-IFIT1 antibody A02652 on canine. You can run a BLAST between canine and the immunogen sequence of anti-IFIT1 antibody A02652 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine epithelium of bronchus in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-02-06

Order DetailsPrice
A02652

100 μL

$335

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