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Product Info Summary
| SKU: | A02265-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IFITM3 Antibody
SKU/Catalog Number
A02265-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-IFITM3 Antibody catalog # A02265-2. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-IFITM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02265-2)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human IFITM3 recombinant protein (Position: M1-Q130).
Reactive Species
A02265-2 is reactive to IFITM3 in Human, Mouse, Rat
Observed Molecular Weight
17 kDa
Calculated molecular weight
14.6 kDa
Background of IFITM3
Interferon-induced transmembrane (IFITM) proteins are a family of interferon induced antiviral proteins. The family contains five members, including IFITM1, IFITM2 and IFITM3 and belong to the CD225 superfamily. The protein encoded by this gene restricts cellular entry by diverse viral pathogens, such as influenza A virus, Ebola virus and Sars-CoV-2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02265-2 is guaranteed for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
Immunoprecipitation, 1:50
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 1:100-1000
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human THP-1 whole cell, human SIHA whole cell
Validation Images & Assay Conditions
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Western blot analysis of IFITM3 using anti-IFITM3 antibody (A02265-2).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFITM3 antigen affinity purified polyclonal antibody (A02265-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IFITM3 at approximately 17 kDa. The expected band size for IFITM3 is at 15 kDa.
Specific Publications For Anti-IFITM3 Antibody (A02265-2)
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