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- Table of Contents
IFT74 antibody (A08931-2) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of JK cells using anti-IFT74 antibody (A08931-2).
Overlay histogram showing JK cells stained with A08931-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IFT74 Antibody (A08931-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing JK cells stained with A08931-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IFT74 Antibody (A08931-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-IFT74 Antibody Picoband®
IFT74 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
IFT74 antibody was positively detected by WB in:
human 293T whole cell, human Jurkat whole cell, human Raji whole cell, monkey COS-7 whole cell, rat thymus tissue, mouse thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
IFT74 antibody was positively detected by IHC in:
human esophageal squamous carcinoma tissue, human lung cancer tissue, human rectum adenocarcinoma tissue, mouse testis tissue, rat testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
IFT74 antibody was positively detected by FC in:
JK cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Tested
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Dog
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-IFT74 Antibody Picoband®
View all IFT74 Antibodies
View all IFT74 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derivedhumanIFT74recombinantprotein(Position:I28-H502).HumanIFT74?shares?89.5%?amino?acid?(aa)?sequence?identity?withmouseIFT74.
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
69 kDa
Calculated Molecular Weight
69.2 kDa
Background of IFT74
Intraflagellar transport protein 74 homolog (IFT74), also known as coiled-coil domain-containing protein 2 (CCDC2) and capillary morphogenesis gene 1 protein (CMG1), is a protein that in humans is encoded by the IFT74 gene. This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis--frontotemporal dementia and Bardet-Biedl Syndrome.
Cite This Product
Anti-IFT74 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08931-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Dog
92%
High Homology
Sheep
91%
High Homology
Rabbit
90%
High Homology
Mouse
89%
Moderate Homology
Rat
89%
Moderate Homology
Cow
87%
Moderate Homology
Zebrafish
61%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for IFT74 antibody
Immunohistochemistry (IHC) protocol for IFT74 antibody
Flow Cytometry (FC) protocol for IFT74 antibody
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Product Promise
We promise Anti-IFT74 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A08931-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026