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Product Info Summary
| SKU: | M00531 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-Ikaros Purified IKZF1 Monoclonal Antibody
SKU/Catalog Number
M00531
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ikaros Purified IKZF1 Monoclonal Antibody (Catalog# M00531). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Mouse, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ikaros Purified IKZF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00531)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
4E9
Isotype
Mouse IgG1
Immunogen
Recombinant human Ikaros (C-terminal part). The mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.
Reactive Species
M00531 is reactive to IKZF1 in Human, Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
57.5 kDa
Background of IKZF1
Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Helios. Ikaros forms homo- and heterodimers with these proteins and functions predominantly in early hematopoietic development. Expression of Ikaros, Aiolos and Helios is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Disruption of Ikaros leads to T and B cell leukemias.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00531 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml. Intracellular staining.
Validation Images & Assay Conditions
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Separation of human monocytes stained using anti-Ikaros (4E9) purified antibody (concentration in sample 8 µg/ml, GAM APC, red-filled) from monocytes stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 8 µg/ml, same as Ikaros purified, GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
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Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Ikaros (4E9) purified antibody (concentration in sample 8 µg/ml, GAM APC).
Specific Publications For Anti-Ikaros Purified IKZF1 Monoclonal Antibody (M00531)
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