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Product Info Summary
| SKU: | M01816 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IKBKE Monoclonal Antibody
SKU/Catalog Number
M01816
BM5656 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IKBKE Monoclonal Antibody catalog # M01816. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKBKE Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01816)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFAO-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IKKi/IKKe Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
Reactive Species
M01816 is reactive to IKBKE in Human
Observed Molecular Weight
80 kDa
Calculated molecular weight
80.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01816 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human Raji whole cell, human Daudi whole cell, human Ramos whole cell, human Hela whole cell, human THP-1 whole cell, human RT4 whole cell, mouse thymus tissue
Validation Images & Assay Conditions
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Western blot analysis of IKBKE using anti-IKBKE antibody (M01816).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human Ramos whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: human RT4 whole cell lysates,
Lane 7: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKE antigen affinity purified monoclonal antibody (M01816) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IKBKE at approximately 80 kDa. The expected band size for IKBKE is at 80 kDa.
Specific Publications For Anti-IKBKE Monoclonal Antibody (M01816)
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Customer Q&As
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2 Customer Q&As for Anti-IKBKE Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01816?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of IKKi/IKKe expression in Raji cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-IKBKE Monoclonal antibody M01816 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The anti-IKBKE Monoclonal antibody (M01816) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-09
