Anti-IKK beta/IKBKB Antibody Picoband™

Product Name

Anti-IKK beta/IKBKB Antibody Picoband™

View all IKK beta Antibodies

SKU/Catalog Number

PB9221

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IKK beta/IKBKB Antibody Picoband™ catalog # PB9221. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IKK beta/IKBKB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9221)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human IKK beta recombinant protein (Position: E398-S756). Human IKK beta shares 91% and 90% amino acid (aa) sequences identity with mouse and rat IKK beta, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9221 is reactive to IKBKB in Human

Applications

PB9221 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

86.564kDa

Background of IKK beta

IKBKB (Inhibitor of Kappa Light Chain Gene Enhancer in B Cells, Kinase of, Beta), also known as IKKB or NFKBIKB, is a protein that in humans is encoded by the IKBKB gene. This gene is mapped to chromosome 8p12-p11 by FISH. It is found that mutations in IKK2 had a more pronounced effect upon NFKB activation than did comparable mutations in IKK1. The activity of various antiinflammatory agents on the IKK complex has been texted, it has been found that aspirin and sodium salicylate specifically inhibit IKK-beta activity in vitro and in vivo by binding to IKK-beta to reduce ATP binding.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Anti-IKK beta antibody, PB9221, Western blotting
All lanes: Anti IKK beta (PB9221) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: M231 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD

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Anti-IKK beta antibody, PB9221,IHC(P)
IHC(P): Human Lung Cancer Tissue

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Anti-IKK beta antibody, PB9221, Western blotting
All lanes: Anti IKK beta (PB9221) at 0.5ug/ml
WB: Recombinant Human IKK beta Protein 0.5ng
Predicted bind size: 43KD
Observed bind size: 43KD

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Figure 4. IF analysis of IKK beta using anti-IKK beta antibody (PB9221).
IKK beta was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IKK beta Antibody (PB9221) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 5. Flow Cytometry analysis of 293T cells using anti-IKK beta antibody (PB9221).
Overlay histogram showing 293T cells stained with PB9221 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK beta Antibody (PB9221,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For IKBKB (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK2; IKK2FLJ38368; IKK-B; IKK-betaFLJ36218; IKKBFLJ40509; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; NFKBIKBFLJ33771; Nuclear factor NF-kappa-B inhibitor kinase beta IKBKB IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB inhibitor of nuclear factor kappa B kinase subunit beta inhibitor of nuclear factor kappa-B kinase subunit beta|I-kappa-B kinase 2|I-kappa-B-kinase beta|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta|nuclear factor NF-kappa-B inhibitor kinase beta|serine/threonine protein kinase IKBKB

For more info on IKBKB, check out the IKBKB Infographic

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In this infographic, you will see the following information for IKBKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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