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Product Info Summary
| SKU: | A01816 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IKK epsilon IKBKE Antibody
SKU/Catalog Number
A01816
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IKK epsilon IKBKE Antibody (Catalog # A01816). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IKK epsilon IKBKE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01816)
Host
Rabbit
Contents
IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IKK epsilon antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IKK epsilon. The immunogen is located within the last 50 amino acids of IKK epsilon.
Cross-reactivity
It has no cross response to IKKa, IKKb, or IKKy.
Reactive Species
A01816 is reactive to IKBKE in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
80.5 kDa
Background of IKBKE
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i. IKK epsilon is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε/IKK-i message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01816 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Induction Validation in Mouse Cell Line
Loading: 15 μg of Raw264.7 cell lysates per lane.
Antibodies: IKK epsilon A01816 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Cells were treated with LPS (0.3 μg/mL) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).
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Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: IKK epsilon A01816 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Mouse Tissue Lysates
Loading: 15 μg of lysates per lane.
Antibodies: IKK epsilon A01816 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Jurkat Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: IKK epsilon A01816 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of IKK epsilon in Human Pancreas Tissue
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IKK epsilon antibody (A01816) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of IKK epsilon in HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling IKK epsilon with A01816 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Specific Publications For Anti-IKK epsilon IKBKE Antibody (A01816)
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