Anti-IKK gamma IKBKG Antibody

Product Name

Anti-IKK gamma IKBKG Antibody

View all IKBKG Antibodies

SKU/Catalog Number

A00874-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-IKK gamma IKBKG Antibody (Catalog # A00874-2). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

IKK gamma antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-IKK gamma IKBKG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00874-2)

Host

Rabbit

Contents

IKK gamma Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

IKK gamma antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IKK gamma. The immunogen is located within the last 50 amino acids of IKK gamma.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

IKK gamma has no cross response to IKK alpha or IKK beta.

Reactive Species

A00874-2 is reactive to IKBKG in Human, Mouse, Rat

Applications

A00874-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

48.198kDa

Background of IKBKG

Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The IκB kinase (IKKα and IKKβ) phosphorylates IkB and mediates NF-κB activation. A novel molecule in the IKK complex was recently identified and termed IKKγ/NEMO/FIP3/IKKAP1. IKKγ interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IKK gamma antibody can be used for detection of IKK gamma by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.

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Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 μg/mL.

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Immunofluorescence of IKK gamma in Hela cells with IKK gamma antibody at 20 μg/mL.

Red: IKK gamma Antibody (A00874-2)
Blue: DAPI staining

Gene/Protein Information For IKBKG (Source: Uniprot.org, NCBI)

Alternative Names

FIP-3IP2; FIP3NF-kappa-B essential modifier; FIP3P; I-kappa-B kinase subunit gamma; IkB kinase gamma subunit; IkB kinase subunit gamma; IkB kinase-associated protein 1; IKBKG; IKK gamma; IKKAP1; IKKG; IKK-gammaIPD2; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IP; IP1; NEMO; NEMOAMCBX1; NFkappaB essential modulator; NF-kappa-B essential modulator IKBKG AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma NF-kappa-B essential modulator|I-kappa-B kinase subunit gamma|NF-kappa-B essential modifier|ikB kinase subunit gamma|ikB kinase-associated protein 1|incontinentia pigmenti|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma|inhibitor of nuclear factor kappa B kinase subunit gamma

For more info on IKBKG, check out the IKBKG Infographic

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In this infographic, you will see the following information for IKBKG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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