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Product Info Summary
| SKU: | A01311 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-IL-17RA Antibody
SKU/Catalog Number
A01311
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IL-17RA Antibody (Catalog # A01311). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
IL-17RA antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IL-17RA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01311)
Host
Rabbit
Contents
IL-17RA Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IL-17RA antibody was raised against an 18 amino acid peptide near the amino terminus of human IL-17RA. The immunogen is located within amino acids 150 - 200 of IL-17RA.
Cross-reactivity
IL-17RA antibody is human, mouse and rat reactive. Multiple isoforms of IL-17RA are known to exist. This antibody is predicted to not cross-react with other IL-17 receptor subunits.
Reactive Species
A01311 is reactive to IL17RA in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
96.1 kDa
Background of IL17RA
Interleukin 17A (IL-17A) is a proinflammatory cytokine secreted by activated T-lymphocytes (1). It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17RA is a ubiquitous type I membrane glycoprotein that binds with low affinity to IL-17A and IL-17F (1,2). IL-17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis (3,4). Defects in IL-17RA are the cause of familial candidiasis type 5 (CANDF5) which is a rare disorder with altered immune responses and impaired clearance of fungal infections (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01311 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC-P/IF: 10-20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Validation Images & Assay Conditions
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WB Validation in K562 Cells
Loading: 10 μg of lysate
Antibodies: IL-17RA, A01311, 4 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation in Mouse Tissues
Loading: 15 μg of lysate
Antibodies: IL-17RA, A01311, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of IL-17RA in Human Spleen
Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling IL-17RA with A01311 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Immunofluorescence Validation of IL-17RA in Mouse Thymus
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17RA with A01311 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Specific Publications For Anti-IL-17RA Antibody (A01311)
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