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Product Info Summary
| SKU: | PA1466 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interleukin-2 IL2 Antibody Picoband®
SKU/Catalog Number
PA1466
BA1645-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-2 IL2 Antibody catalog # PA1466. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-2 IL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1466)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL-2.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1466 is reactive to Il2 in Mouse
Observed Molecular Weight
24 kDa
Calculated molecular weight
19.4 kDa
Background of Il2
IL-2, Interleukin-2, formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin-or antigen-activated T cells. In situ hybridization, the IL-2 gene is assigned to 4q26-q28. IL2 can act as a growth hormone for both B and T lymphocytes. It is useful in the study of the molecular nature of T-cell differentiation and, like interferons, augments natural killer cell activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1466 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Anti-IL-2 antibody, PA1466, Western blotting
Lane 1: Recombinant Mouse IL-2 Protein 10ng
Lane 2: Recombinant Mouse IL-2 Protein 5ng
Lane 3: Recombinant Mouse IL-2 Protein 2.5ng
Specific Publications For Anti-Interleukin-2 IL2 Antibody Picoband® (PA1466)
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Customer Q&As
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3 Customer Q&As for Anti-Interleukin-2 IL2 Antibody Picoband®
Question
We are currently using anti-IL2 antibody PA1466 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-IL2 antibody (PA1466) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
We have observed staining in mouse blood. Do you have any suggestions? Is anti-IL2 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
From literature blood does express IL2. From Uniprot.org, IL2 is expressed in small intestine peyer's patch, placenta, blood, among other tissues. Regarding which tissues have IL2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Placenta, Pubmed ID: 8824916
Boster Scientific Support
Answered: 2019-11-14
Question
We were happy with the WB result of your anti-IL2 antibody. However we have observed positive staining in small intestine peyer's patch secreted. using this antibody. Is that expected? Could you tell me where is IL2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
Based on literature, small intestine peyer's patch does express IL2. Generally IL2 expresses in secreted. Regarding which tissues have IL2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Placenta, Pubmed ID: 8824916
Boster Scientific Support
Answered: 2019-10-11
