Anti-IL2 Antibody Picoband™

Product Name

Anti-IL2 Antibody Picoband™

View all IL-2 Antibodies

SKU/Catalog Number

PB9013

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL2 Antibody Picoband™ catalog # PB9013. Tested in WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9013)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat IL-2 recombinant protein (Position: A21-Q155). Rat IL-2 shares 66% and 80% amino acid (aa) sequences identity with human and mouse IL-2, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9013 is reactive to IL2 in Rat

Applications

PB9013 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

18 kDa

Calculated molecular weight

17.628kDa

Background of IL-2

Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. This gene for interleukin 2 (IL2) is assigned to chromosome 4. IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of IL2 using anti-IL2 antibody (PB9013).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Thymus Tissue Lysate,
Lane 2: Rat Liver Tissue Lysate,
Lane 3: NRK Whole Cell Lysate,
Lane 4: PC12 Whole Cell Lysate,
Lane 5: RH35 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2 antigen affinity purified polyclonal antibody (Catalog # PB9013) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2 at approximately 18KD. The expected band size for IL2 is at 18KD.

Gene/Protein Information For IL2 (Source: Uniprot.org, NCBI)

Alternative Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF IL2 IL-2, TCGF, lymphokine interleukin 2 interleukin-2|T cell growth factor|aldesleukin|involved in regulation of T-cell clonal expansion

For more info on IL2, check out the IL2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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