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- Table of Contents
Il2 antibody (PB9013) performs well in WB and shows reactivity with Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Western blot analysis of IL2 using anti-IL2 antibody (PB9013).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Thymus Tissue Lysate,
Lane 2: Rat Liver Tissue Lysate,
Lane 3: NRK Whole Cell Lysate,
Lane 4: PC12 Whole Cell Lysate,
Lane 5: RH35 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2 antigen affinity purified polyclonal antibody (Catalog # PB9013) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2 at approximately 18KD. The expected band size for IL2 is at 18KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Thymus Tissue Lysate,
Lane 2: Rat Liver Tissue Lysate,
Lane 3: NRK Whole Cell Lysate,
Lane 4: PC12 Whole Cell Lysate,
Lane 5: RH35 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2 antigen affinity purified polyclonal antibody (Catalog # PB9013) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2 at approximately 18KD. The expected band size for IL2 is at 18KD.
Designing a Western blot for IL2? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the IL2 Western Blot GuideBundle Save
Tested Applications
Il2 antibody was positively detected by WB in:
Rat Thymus Tissue, Rat Liver Tissue, NRK Whole Cell, PC12 Whole Cell, RH35 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IL2 Antibody Picoband®
View all IL2 Antibodies
View all IL2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat IL-2 recombinant protein (Position: A21-Q155). Rat IL-2 shares 66% and 80% amino acid (aa) sequences identity with human and mouse IL-2, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated Molecular Weight
17.6 kDa
Background of Il2
Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. This gene for interleukin 2 (IL2) is assigned to chromosome 4. IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
Cite This Product
Anti-IL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9013)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
80%
Moderate Homology
Human
66%
Low homology
Monkey
66%
Low homology
Rabbit
63%
Low homology
Dog
61%
Low homology
Sheep
51%
Low homology
Cow
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Il2 antibody
Publications
Application:
Species:
Selenium deficiency modulates necroptosis-mediated intestinal inflammation in broiler through the lncRNAWSF27/miRNA1696/GPX3 axis
Published: 2024-09-27
Journal: JOURNAL OF ANIMAL SCIENCE
Application: WB
Impact Factor: 2.70
Species: Chicken
PMID: 39331000
Effects of Weizhuan’an on rats with precancerous lesions of gastric cancer based on regulating gastric mucosal microflora and inflammatory factors
Published: 2024-08-16
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.40
Species: Rat
PMID: 39221149
miR-210/NF-κB axis: A new direction for regulating cadmium-induced pig artery inflammatory injury
Published: 2023-06-03
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 5.60
Species: Pig
PMID: 37269461
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Product Promise
We promise Anti-IL2 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9013
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 18, 2026