Anti-IL-21 Antibody

Product Name

Anti-IL-21 Antibody

View all IL-21 Antibodies

SKU/Catalog Number

A00459

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-IL-21 Antibody (Catalog # A00459). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

IL-21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-IL-21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00459)

Host

Rabbit

Contents

IL-21 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-IL-21 antibody was raised against a peptide corresponding to 15 amino acids near the center of human IL-21 precursor. The immunogen is located within the last 50 amino acids of IL-21.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.

Reactive Species

A00459 is reactive to IL21 in Human, Mouse, Rat

Applications

A00459 is guaranteed for ELISA, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

18.653kDa

Background of IL-21

A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1 μg/mL; ICC: 2 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western Blot Validation in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: IL-21 A00459, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: IL-21 A00459 (1 μg/mL), IL-21 (5 μg/mL), beta-actin (5 μg/mL) and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Human HL-60 Cell Lysate
Loading: 15 μg of lysates per lane. Antibodies: IL-21 A00459 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.

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Immunocytochemistry Validation of IL-21 in K562 Cells
Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (A00459) at 2 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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Induced Expression Validation of IL-21 expresssion in patients with Crohn’s Disease (Monteleone et al

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Induced Expression Validation of IL-21 expresssion in patients with Helicobacter Pylori (Hp) (Caruso et al.

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Regulation of IL-21 expresssion in duodenal biopsies of two patients with active Celiac disease (ACD) (Sarra et al.