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Product Info Summary
| SKU: | A00857 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC |
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Product info
Product Name
Anti-IL-27A Antibody
SKU/Catalog Number
A00857
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IL-27A Antibody catalog # A00857. Tested in IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-27A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00857)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 1-50
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00857 is reactive to IL27 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
27.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00857 is guaranteed for ELISA, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:50-200
ELISA 1:10000-20000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.
Specific Publications For Anti-IL-27A Antibody (A00857)
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Customer Q&As
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14 Customer Q&As for Anti-IL-27A Antibody
Question
I was wanting to use your anti-IL-27A antibody for IHC for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
You can see on the product datasheet, A00857 anti-IL-27A antibody has been tested for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-17
Question
Will A00857 anti-IL-27A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
It shows on the product datasheet, A00857 anti-IL-27A antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-17
Question
We are currently using anti-IL-27A antibody A00857 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The anti-IL-27A antibody (A00857) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
Question
My colleagues were well pleased with the WB result of your anti-IL-27A antibody. However we have seen positive staining in right lobe of liver secreted. using this antibody. Is that expected? Could you tell me where is IL27 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
Based on literature, right lobe of liver does express IL27. Generally IL27 expresses in secreted. Regarding which tissues have IL27 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-28
Question
I am looking for to test anti-IL-27A antibody A00857 on human lung for research purposes, then I may be interested in using anti-IL-27A antibody A00857 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
The products we sell, including anti-IL-27A antibody A00857, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-30
Question
See below the WB image, lot number and protocol we used for lung using anti-IL-27A antibody A00857. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-09
Question
Is this A00857 anti-IL-27A antibody reactive to the isotypes of IL27?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
The immunogen of A00857 anti-IL-27A antibody is Synthetic peptide from human protein at AA range: 1-50. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-17
Question
Is there a BSA free version of anti-IL-27A antibody A00857 available?
E. Patel
Verified customer
Asked: 2018-04-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-27A antibody A00857 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-30
Question
I see that the anti-IL-27A antibody A00857 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-06
Question
Can you help my question with product A00857, anti-IL-27A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Taylor
Verified customer
Asked: 2017-11-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00857 anti-IL-27A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-15
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-IL-27A antibody A00857. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-22
Question
We have seen staining in mouse lung. Are there any suggestions? Is anti-IL-27A antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
According to literature lung does express IL27. According to Uniprot.org, IL27 is expressed in right lobe of liver, lung, among other tissues. Regarding which tissues have IL27 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-06-15
Question
Is a blocking peptide available for product anti-IL-27A antibody (A00857)?
T. Walker
Verified customer
Asked: 2016-05-06
Answer
We do provide the blocking peptide for product anti-IL-27A antibody (A00857). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-05-06
Question
Does anti-IL-27A antibody A00857 work for IHC with lung?
D. Thomas
Verified customer
Asked: 2014-01-02
Answer
According to the expression profile of lung, IL27 is highly expressed in lung. So, it is likely that anti-IL-27A antibody A00857 will work for IHC with lung.
Boster Scientific Support
Answered: 2014-01-02
