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Product Info Summary
| SKU: | A09233 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-IL-28 IFNL2 Antibody
SKU/Catalog Number
A09233
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IL-28 IFNL2 Antibody catalog # A09233. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-28 IFNL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09233)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human IL28A. AA range:131-180
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09233 is reactive to IFNL2 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
22.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09233 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of MCF7 cells using IL-28 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from MCF7 cells, using IL28A Antibody.
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Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:100
Specific Publications For Anti-IL-28 IFNL2 Antibody (A09233)
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Customer Q&As
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6 Customer Q&As for Anti-IL-28 IFNL2 Antibody
Question
We want to test anti-IL-28 antibody A09233 on human peripheral blood leukocyte for research purposes, then I may be interested in using anti-IL-28 antibody A09233 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The products we sell, including anti-IL-28 antibody A09233, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-19
Question
Will anti-IL-28 antibody A09233 work on monkey IHC with epithelium of nasopharynx?
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
Our lab technicians have not tested anti-IL-28 antibody A09233 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-IL-28 antibody A09233 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey epithelium of nasopharynx in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-24
Question
Would A09233 anti-IL-28 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Jackson
Verified customer
Asked: 2017-11-27
Answer
As indicated on the product datasheet, A09233 anti-IL-28 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-27
Question
Do you have a BSA free version of anti-IL-28 antibody A09233 available?
H. Carter
Verified customer
Asked: 2017-07-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-28 antibody A09233 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-03
Question
I see that the anti-IL-28 antibody A09233 works with IHC, what is the protocol used to produce the result images on the product page?
R. Zhang
Verified customer
Asked: 2016-05-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-05-11
Question
Can you help my question with product A09233, anti-IL-28 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Kulkarni
Verified customer
Asked: 2013-02-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09233 anti-IL-28 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-11
