Anti-Interleukin-3 IL3 Antibody

Product Name

Anti-Interleukin-3 IL3 Antibody

View all IL-3 Antibodies

SKU/Catalog Number

PA1728

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-3 IL3 Antibody catalog # PA1728. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-3 IL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1728)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse IL-3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1728 is reactive to IL3 in Mouse

Applications

PA1728 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

14 kDa

Calculated molecular weight

17.233kDa

Background of IL-3

Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. Ot is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

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Anti-IL-3 antibody, PA1728, Western blotting
All lanes: Anti IL-3 (PA1728) at 0.5ug/ml
Lane 1: Recombinant Mouse IL-3 Protein 10ng
Lane 2: Recombinant Mouse IL-3 Protein 5ng
Lane 3: Recombinant Mouse IL-3 Protein 2.5ng
Predicted bind size: 14KD
Observed bind size: 14KD

Gene/Protein Information For IL3 (Source: Uniprot.org, NCBI)

Alternative Names

Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor IL3 IL-3, MCGF, MULTI-CSF interleukin 3 interleukin-3|P-cell stimulating factor|colony-stimulating factor, multiple|hematopoietic growth factor|mast-cell growth factor|multilineage-colony-stimulating factor|multipotential colony-stimulating factor

For more info on IL3, check out the IL3 Infographic

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In this infographic, you will see the following information for IL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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