Product Info Summary
SKU: | M06903 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-IL-34 Monoclonal Antibody
SKU/Catalog Number
M06903
Size
100ul
Form
Liquid
Description
Boster Bio Anti-IL-34 Monoclonal Antibody catalog # M06903. Tested in ELISA, Flow Cytometry, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-34 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06903)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1D12
Isotype
IgG
Immunogen
Purified recombinant fragment of human IL-34 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06903 is reactive to Il34 in Human
Applications
M06903 is guaranteed for ELISA, Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
26.764kDa
Background of IL-34
Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Flow Cytometry validation of IL34 using Anti-IL-34 Monoclonal Antibody (M06903).
Flow cytometric (FCM) analysis of K562 cells using IL-34 Monoclonal Antibody (green) and negative control (purple).Click image to see more details
Figure 2. Immunofluorescent staining data of IL34 using Anti-IL-34 Monoclonal Antibody (M06903).
Immunofluorescence (IF) analysis of U251 cells using IL-34 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Click image to see more details
Figure 1. Western blotting validation for Anti-IL-34 Monoclonal Antibody M06903
Western Blot (WB) analysis using IL-34 Monoclonal Antibody against IL-34-hIgGFc transfected HEK293 cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For Il34 (Source: Uniprot.org, NCBI)
Gene Name
Il34
Full Name
Interleukin-34
Weight
26.764kDa
Superfamily
IL-34 family
Alternative Names
C16orf77; IL34; IL-34; interleukin 34 Il34|2010004A03Rik, AI593503|interleukin 34|interleukin-34
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il34, check out the Il34 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-34 Monoclonal Antibody (M06903)
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No publications found for M06903
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Customer Reviews
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Customer Q&As
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