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Product Info Summary
| SKU: | A05759 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IL-36G Antibody
SKU/Catalog Number
A05759
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IL-36G Antibody (Catalog # A05759). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
IL-36G antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-IL-36G Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05759)
Host
Rabbit
Contents
IL-36G antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IL-36G antibody was raised against a 13 amino acid peptide near the carboxy terminus of human IL-36G. The immunogen is located within the last 50 amino acids of IL-36G.
Cross-reactivity
IL-36G antibody is human specific. IL-36G antibody will not cross-react with IL-36A or IL-36B.
Reactive Species
A05759 is reactive to IL36G in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
18.7 kDa
Background of IL36G
IL-36G is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36G is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2). Like the related proteins IL-36A and IL-36B, IL-36G requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown (3). IL-36G expression is stimulated by interferon-gamma, tumor necrosis factor-alpha and interleukin 1 (4). The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05759 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IL-36G antibody can be used for detection of IL-36G by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IL-36G in human spleen tissue lysate with IL-36G antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of IL-36G in human bladder tissue with IL-36G antibody at 5 μg/mL.
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Immunofluorescence of IL-36G in human bladder tissue with IL-36G antibody at 20 μg/mL.
Specific Publications For Anti-IL-36G Antibody (A05759)
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