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Product Info Summary
| SKU: | A02925-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IL-9 Antibody
SKU/Catalog Number
A02925-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IL-9 Antibody (Catalog # A02925-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
IL-9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-IL-9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02925-1)
Host
Rabbit
Contents
IL-9 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
Anti-IL-9 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IL-9. The immunogen is located within the last 50 amino acids of IL-9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
IL-9 antibody is human specific. IL-9 antibody is predicted to no cross-react with IL-7.
Reactive Species
A02925-1 is reactive to IL9 in Human
Applications
A02925-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
15.909kDa
Background of IL-9
Interleukin 9 (IL-9) is a cytokine secreted by TH2 lymphocytes that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation and prevents apoptosis (1,2). It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins (3). The IL-9 gene has been identified as a candidate gene for asthma (4). Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human Spleen Tissue Lysate
Loading: 15 μg of lysates per lane.Antibodies: IL-9, A02925-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation with Recombinant Protein
Loading: 30 ng of human IL-9 recombinant protein per lane.Antibodies: IL-9, A02925-1 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 22kD.
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Western Blot Validation in Tissue and Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: IL-9, A02925-1 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of IL-9 in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (A02925-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of IL-9 in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (A02925-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Protein Target Info & Infographic
Gene/Protein Information For IL9 (Source: Uniprot.org, NCBI)
Gene Name
IL9
Full Name
Interleukin-9
Weight
15.909kDa
Superfamily
IL-7/IL-9 family
Alternative Names
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40 IL9 HP40, IL-9, P40 interleukin 9 interleukin-9|T-cell growth factor p40|cytokine P40|homolog of mouse T cell and mast cell growth factor 40|p40 T-cell and mast cell growth factor|p40 cytokine
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL9, check out the IL9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-9 Antibody (A02925-1)
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No publications found for A02925-1
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Customer Reviews
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