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- Table of Contents
Il1rn antibody (A00651-5) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HEPA1-6 cells using anti-IL1RA/Il1rn antibody (A00651-5).
Overlay histogram showing HEPA1-6 cells stained with A00651-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL1RA/Il1rn Antibody (A00651-5, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPA1-6 cells stained with A00651-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL1RA/Il1rn Antibody (A00651-5, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-IL1RA/Il1rn Antibody Picoband®
Il1rn antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
Il1rn antibody was positively detected by WB in:
rat spleen tissue, rat brain tissue, rat thymus tissue, rat kidney tissue, rat PC-12 whole cell, mouse spleen tissue, mouse kidney tissue, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Il1rn antibody was positively detected by FC in:
HEPA1-6 cell, NRK cell
Il1rn antibody was positively detected by IHC in:
mouse lung tissue, rat lung tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-IL1RA/Il1rn Antibody Picoband®
View all IL1RN Antibodies
View all IL1RN Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse IL1RA/Il1rn recombinant protein (Position: K35-Q178).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated Molecular Weight
20.3 kDa
Background of Il1rn
The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
Cite This Product
Anti-IL1RA/Il1rn Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00651-5)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
91%
High Homology
Dog
79%
Moderate Homology
Human
78%
Moderate Homology
Rabbit
78%
Moderate Homology
Cow
78%
Moderate Homology
Monkey
78%
Moderate Homology
Sheep
76%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Il1rn antibody
Flow Cytometry (FC) protocol for Il1rn antibody
Immunohistochemistry (IHC) protocol for Il1rn antibody
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Product Promise
We promise Anti-IL1RA/Il1rn Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00651-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026