Anti-IL1RA/Il1rn Antibody Picoband™

Product Name

Anti-IL1RA/Il1rn Antibody Picoband™

See all IL-1ra/IL-1F3/IL1RN products

SKU/Catalog Number

A00651-4

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-IL1RA/Il1rn Antibody Picoband™ catalog # A00651-4. Tested in WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL1RA/Il1rn Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00651-4)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Immunogen

E. coli-derived rat IL1RA recombinant protein (Position: H27-Q178). Rat IL1RA shares 77.6% and 90.7% amino acid (aa) sequence identity with human and mouse IL1RA, respectively.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00651-4 is reactive to Il1rn in Rat

Applications

A00651-4 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of IL1RA using anti-IL1RA antibody (A00651-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA antigen affinity purified polyclonal antibody (Catalog # A00651-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1RA at approximately 28KD. The expected band size for IL1RA is at 20KD.

Gene/Protein Information For Il1rn (Source: Uniprot.Org, NCBI)

Alternative Names

DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 Il1rn|IL-1ra|interleukin 1 receptor antagonist|interleukin-1 receptor antagonist protein|IL-1RN|IL1 inhibitor|IRAP

For more info on Il1rn, check out the Il1rn Infographic

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In this infographic you will see the following information for Il1rn: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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