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Product Info Summary
| SKU: | A00807-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-IL4R Antibody (C-term)
SKU/Catalog Number
A00807-3
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-IL4R Antibody (C-term) (Catalog # A00807-3). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-IL4R Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00807-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This IL4R antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 677-691 amino acids from the C-terminal region of human IL4R.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00807-3 is reactive to IL4R in Human
Calculated molecular weight
89.7 kDa
Background of IL4R
Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00807-3 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody (C-term). A00807-3 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Flow cytometric analysis of Ramos cells using IL4R Antibody (C-term)(green, ) compared to an isotype control of rabbit IgG(blue). A00807-3 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-IL4R Antibody (C-term) (A00807-3)
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