Anti-ILP-2 BIRC8 Antibody

Product Name

Anti-ILP-2 BIRC8 Antibody

View all BIRC8 Antibodies

SKU/Catalog Number

A10326

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ILP-2 BIRC8 Antibody (Catalog # A10326). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ILP-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ILP-2 BIRC8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10326)

Host

Rabbit

Contents

ILP-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ILP-2 antibody was raised with against a synthetic peptide corresponding to amino acids near the amino terminus of human ILP-2. The immunogen is located within the first 50 amino acids of ILP-2.

Reactive Species

A10326 is reactive to BIRC8 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

27.1 kDa

Background of BIRC8

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2. ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues. ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10326 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ILP-2 antibody can be used for detection of ILP-2 by Western blot at 1 μg/mL. A band at approximately 33 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BIRC8

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